单核细胞趋化蛋白1与神经干细胞的体外迁移

摘要

BACKGROUND: Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells (NSCs) migration.OBJECTIVE: To explore the effect of monocyte chemoattractant protein 1 (MCP-1) and its receptoron the migrati on ofNSCs in vitro.METHODS: Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified andidentified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 withdifferent concentrations (50, 100, 200, 300, 500 ng/mL) on NSCs in vitro migration was obsened under agarose.RESULTS AND CONCLUSION: Immunofluorescence and RT-PCR results showed that NSCs from fetal rathippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that theMCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the betterthe migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs.%背景:研究表明趋化因子基质细胞衍生因子1及血管内皮生长因子参与神经干细胞的迁移.目的:观察趋化因子单核细胞趋化蛋白1及其受体CCR2对神经干细胞体外迁移的作用.方法:分离培养胎鼠海马组织神经干细胞,体外传代扩增及鉴定;通过细胞免疫荧光及RT-PCR检测其受体CCR2表达情况;通过琼脂糖下细胞迁移实验观察50,100,200,300,500 μg/L 单核细胞趋化蛋白1对神经干细胞的体外趋化作用.结果与结论:细胞免疫荧光及RT-PCR检测证实胎鼠海马来源神经干细胞表达趋化因子受体CCR2;琼脂糖下细胞迁移实验显示,体外条件下单核细胞趋化蛋白1可趋化神经干细胞迁移,且趋化迁移作用随质量浓度增加而增强,抗CCR2多克隆抗体可对抗其趋化迁移作用.