背景 基质金属蛋白酶在椎间盘退变中通过影响基质合成及降解导致基质成分紊乱及功能的改变.目的 观察基质金属蛋白酶1 在人类退变腰椎间盘组织中的表达变化.方法 获取经手术治疗腰椎间盘突出症患者椎间盘标本30 份,突出型、脱出型、游离型各10 份,分离髓核与纤维环,设为实验组;腰椎损伤致椎体骨折切除椎间盘10 份设为对照组.应用免疫组织化学法检测基质金属蛋白酶1 在椎间盘中髓核与纤维环细胞中的表达.结果与结论 ①苏木精-伊红染色显示,对照组椎间盘标本纤维环仍保持着致密的板层结构,实验组椎间盘组织多呈纤维化样改变.②两组髓核细胞中基质金属蛋白酶1 表达均高于纤维环细胞中的表达.③实验组突出型椎间盘标本中基质金属蛋白酶1 表达明显高于对照组(P < 0.01),脱出型和游离型椎间盘标本中基质金属蛋白酶1 表达明显高于突出型 (P < 0.01).%BACKGROUND: Matrix metalloproteinases lead to matrix components disorders and change the function through the influence on matrix synthesis and degradation in the disc degeneration.OBJECTIVE: To observe the expression of matrix metalloproteinase-1 (MMP-1) in degenerative human intervertebral disc.METHODS: Thirty intervertebral disk samples were selected from the prolapse of lumbar intervertebral disc patients in surgery.Experimental group was made from 30 cases with lumbar disc protrusion disease and control group was made from 10 cases of lumbar spine fracture in traumatic. Experimental group included protrusion, extrusion and sequestration type, 10 samples in each type and the nucleus pulposus and annulus were separated. The immunohistochemistry was used to detect the expression of MMP-1 in nucleus cells and fiber ring.RESULTS AND CONCLUSION: ①Hematoxylin-eosin staining showed that intervertebral disc specimen fiber ring still maintained a dense lamellar structure in the control group and the intervertebral disc tissue present fibrotic changes in the experimental group.②The expression of MMP-1 in nucleus cells of two groups were higher than those in the fibrous ring. ③The expression of MMP-1in the protrusion specimen of experimental group was significantly higher than that of control group (P < 0.01), and MMP-1expression in extrusion type and sequestration type specimen was significantly higher than that in the protrusion specimen (P <0.01).
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