人脐带间充质干细胞腹腔移植治疗急性肝损伤大鼠

摘要

BACKGROUND:In vitro experiments have confirmed that human umbilical cord mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells, thus which can be considered to function as liver repair. OBJECTIVE:To observe the therapeutic effect of human umbilical cord mesenchymal stem celltransplantation on acute liver injuries in rats through in vivo animal experiments. METHODS:Healthy Sprague-Dawley rats were randomly divided into three groups:normal control group without modeling, celltransplantation group, and PBS group. Rat models of acute liver injury were prepared by 10%CCl4-olive oil solution in the celltransplantation and PBS groups which were fol owed by intraperitoneal injection of 0.5 mL human umbilical cord mesenchymal stem cellsuspension and 0.5 mL PBS, respectively. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that pathological changes related to acute liver injury appeared at 24 hours after intraperitoneal injection of CCl 4 . Then, the liver structure recovered at 7 days after celltransplantation, but it did not recover til the 14th day after PBS injection. Compared with the normal control group, serum alanine aminotransferase and aspartate aminotransferase levels were significantly increased in the other two groups (P<0.05-0.01). In the celltransplantation group, the serum alanine aminotransferase and aspartate aminotransferase levels were significantly lower than those in the PBS group at 3 days after treatment (P<0.05-0.01), and recovered normal y after 7 days. cells positive for anti-human nucleoprotein antibody were found in the portal area of liver tissues in the celltransplantation group after 3 days of transplantation, and then cells positive for anti-human albumin antibody appeared after 7 days. These findings indicate that intraperitoneal transplantation of human umbilical cord mesenchymal stem cells can improve liver function and repair injured liver tissues after acute liver injury in rats to some extent.%背景：体外实验证实人脐带间充质干细胞可诱导成肝样细胞，故认为其可能具有肝脏修复功能。　　目的：进行动物体内实验，观察人脐带间充质干细胞移植治疗大鼠急性肝损伤的效果。　　方法：将健康SD大鼠随机分为3组，正常对照组不造模，细胞移植组和PBS组腹腔注射体积分数10%CCl4橄榄油溶液制造急性肝损伤模型后24 h，分别经腹腔移植人脐带间充质干细胞悬液0.5 mL和等量PBS。　　结果与结论：苏木精-伊红染色显示，CCl4腹腔注射24 h后大鼠肝脏出现急性肝损伤的病理变化，细胞移植后7 d时肝脏结构完全恢复正常，PBS组14 d才恢复正常肝脏组织结构。与正常对照组相比，其他2组大鼠血清丙氨酸转氨酶及天门冬氨酸转氨酶均明显升高(P<0.05-0.01)；人脐带间充质干细胞移植后3 d，大鼠血清2种转氨酶水平较PBS组明显降低(P<0.05-0.01)；7 d后均降至正常水平。移植后第3天肝脏组织内可见抗人核蛋白抗体阳性细胞分布于汇管区，第7天抗人白蛋白抗体的阳性表达。提示经鼠腹腔移植人脐带间充质干细胞后可以在一定程度上改善肝功能及修复受损的肝组织。