运动诱导骨骼肌线粒体生成中沉默信息调节因子1的作用**☆

摘要

背景：沉默信息调节因子1是新近受到体育科学领域关注的能量代谢调节因子，在运动骨骼肌线粒体生成中与其他信号分子一起发挥作用。　　目的：综述沉默信息调节因子1在运动诱导骨骼肌线粒体生物合成中的作用及机制。　　方法：应用计算机检索PubMed和Highwire数据库2000年1月至2013年1月有关运动、沉默信息调节因子1、骨骼肌线粒体生物合成的文献，检索词为SIRT1，AMPK，PGC-1α，mitochondrial biogenesis，skeletal muscle, exercise，限定文章语言为English。对资料进行初审，选取沉默信息调节因子1与运动诱导骨骼肌线粒体生物合成有关的文献，排除重复研究。　　结果与结论：共收集相关文献165篇，排除重复研究，纳入62篇。沉默信息调节因子1作为一种NAD+依赖的去乙酰化酶，在运动中被激活，通过上调过氧化物酶体增殖物激活受体辅激活因子表达而诱导骨骼肌线粒体生物合成，其分子机制涉及一磷酸腺苷激活的蛋白激酶、低氧诱导因子2α等信号分子。但近年来的一些研究对沉默信息调节因子1在骨骼肌线粒体生物合成中的作用提出了质疑，认为其并非为运动诱导的骨骼肌线粒体生物合成所必需。沉默信息调节因子1在运动诱导的骨骼肌线粒体生物合成中发挥重要调控作用，但采用蛋白和活性等不同检测方法，在实验结果上可能会造成较大差异。%BACKGROUND:Silent information regulator factor-1 is an energy metabolism regulator newly received attention in sports science, which playing roles in skeletal exercise-induced muscle mitochondrial biogenesis with other regulatory factors. OBJECTIVE: To review the effect and mechanism of silent information regulator factor-1 on skeletal muscle mitochondrial biogenesis in exercise. METHODS:The PubMed database and Highwire database were retrieved with computer for the articles on exercise, silent information regulator factor-1 and skeletal muscle mitochondrial biogenesis from January 2000 to January 2013 with the key words of“SIRT1, AMPK, PGC-1α, mitochondrial biogenesis, skeletal muscle, exercise”in English. After primary search, the articles about the association between silent information regulator factor-1 and skeletal muscle mitochondrial biogenesis in exercise were selected. Articles on repeated experiment were excluded. RESULTS AND CONCLUSION:Total y 165 relevant articles were selected, and articles on repetitive research were excluded, so finaly 62 articles were included. As a NAD+-depended deacetylase, silent information regulator factor-1 induced skeletal muscle mitochondrial biogenesis by up-regulated peroxisome proliferator-activated receptor coactivator after activated during exercise. The molecular mechanism involved adenosine monophosphate-activated protein kinase and hypoxia-inducible factor 2α. In recent years, the effect of silent information regulator factor-1 on skeletal muscle mitochondrial biogenesis was doubt, the researchers though that silent information regulator factor-1 was not required for exercise-induced muscle mitochondrial biogenesis. Silent information regulator factor-1 plays an important role in exercise-induced muscle mitochondrial biogenesis. But protein and activity detection methods are different in experimental results.