背景：磁性复合骨水泥作为一种新型骨转移治疗材料，不仅能对骨缺损进行修复，还能在交变磁场下升温杀死骨肿瘤。目的：检测磁性复合骨水泥对小鼠L929成纤维细胞增殖活性的影响及细胞毒性反应。方法：采用CCK-8法检测含0%(即磷酸钙骨水泥组)，10%，20% Fe3O4的磁性磷酸钙骨水泥浸提液及含0%(即聚甲基丙烯酸甲酯骨水泥组)，10%，20%Fe3O4的聚甲基丙烯酸甲酯骨水泥浸提液对对数生长期小鼠L929成纤维细胞的毒性作用，每种浸提液又分为50%与100%两个浓度梯度；以正常培养基培养的小鼠L929成纤维细胞作为阴性对照组。结果与结论：①在100%浓度梯度下：除含20%Fe3O4的磁性聚甲基丙烯酸甲酯骨水泥组细胞数量较少、细胞形态异常外，其余磁性聚甲基丙烯酸甲酯骨水泥组细胞生长状态良好；含10%Fe3O4的磁性磷酸钙骨水泥组培养24 h时表现出轻度细胞毒性，毒性2级；含20%Fe3O4的聚甲基丙烯酸甲酯骨水泥在24-72 h为表现出轻、中度细胞毒性，毒性为2，3级。各磁性磷酸钙骨水泥组细胞数量及形态正常。②在50%浓度梯度下：各组细胞形态均正常，细胞毒性为0级或1级。表明磁性聚甲基丙烯酸甲酯骨水泥具有轻、中度细胞毒性，磁性磷酸钙骨水泥细胞相容性良好，基本无细胞毒性。%BACKGROUND: Magnetic bone cement has been used as a new kind of material for bone metastasis, and it can repair bone defects and cure bone tumors with temperature rising under an alternating magnetic field. OBJECTIVE: To test the magnetic bone cement effects on cytotoxicity and cell proliferation of mouse L929 cells. METHODS: The cytotoxicity of magnetic calcium phosphate cement and polymethylmethacrylate bone cement leaching liquors, respectively, containing 0, 10%, 20% Fe3O4, to L929 cells at logarithmic growth phase was detected using Cell Count Kit-8 assay. Two kinds of leaching liquors were prepared to two concentration gradients, 50% and 100%. Mouse L929 cells cultured in normal culture medium were considered as control group. RESULTS AND CONCLUSION: (1) Under 100% concentration gradient: Cell s grew wel in polymethyl methacrylate bone cement groups containing 0, 10% Fe3O4 except for 20% Fe3O4. Magnetic calcium phosphate cement containing 10% Fe3O4 showed mild cell toxicity, graded as 2, after culture for 24 hours. During 24- 72 hours, polymethylmethacrylate bone cement containing 20% Fe3O4 exhibited mild or moderate cell toxicity and the toxicity was graded as 2 or 3. (2) Under 50 concentration gradient: Cells grew wel in al groups. The cell toxicity was graded as 0 or 1. Magnetic polymethylmethacrylate bone cement has mild or moderate cell toxicity and magnetic calcium phosphate cement exhibits a good biocompatibility with no cell toxicity.
展开▼
