RNAⅢ抑制肽抑制葡萄球菌对Hela细胞的黏附

摘要

BACKGROUND:Staphylococcal infections and its biofilm formation can occur when orthopedic implants or wound is healing, and are regulated by bacterial population sensing mechanism. RNAIII inhibiting peptide intervenes the quorum-sensing system of staphylococcal and blocks the signal transduction among staphylococcal cells, and inhibits staphylococcal biofilm formation, and then prevents staphylococcal infections. OBJECTIVE:To investigate the influence of RNAIII inhibiting peptide on the adhesion of staphylococcus epidermis to the Hela cells. METHODS:The Hela cells were cultured in vitro. There were four groups in this study. In the blank group, saline with dimethyl sulfoxide was added in each wel . In the RNAIII inhibiting peptide group, dimethyl sulfoxide solution containing RNAIII inhibiting peptide was added. In the levofloxacin group, levofloxacin was added. In the combination group, the dose was in accordance with above methods. Using intergroup control method, the adhesion of staphylococcus epidermis to the Hela cells was compared under the effects of saline, RNAIII inhibiting peptide and levofloxacin and their combination. RESULTS AND CONCLUSION:In the blank group, abundant bacterial adhered to Hela cells. The number of adhered bacteria was significantly lower in each medicine group than in the blank group (P<0.001). The spot count was significantly lower in the levofloxacin group than in the RNAIII inhibiting peptide group (P<0.05). In the combination group, the number of bacteria adhered to Hela cells was decreased (P<0.01). Results verified that RNAIII inhibiting peptide effectively suppressed the adhesion of staphylococcus epidermis to the host cells, and showed synergistic effects on antibiotics.%背景：葡萄球菌导致的细菌感染和生物被膜形成可在骨科植入物或创口愈合时发生。受细菌群体感应机制调控，葡萄球菌RNAⅢ抑制肽可以干预葡萄球菌的群体感应系统，阻断葡萄球菌细胞间的信号传导通路，从而抑制葡萄球菌的生物被膜形成，防止葡萄球菌感染。　　目的：观察RNAⅢ抑制肽抑制表皮葡萄球菌对人宫颈癌上皮细胞黏附的效果。　　方法：体外培养人宫颈癌上皮细胞，实验分4组，空白组每孔加入DMSO的生理盐水，RNAⅢ抑制肽组加含RNAⅢ抑制肽的DMSO溶液，左氧组加入左氧氟沙星的水溶液，联合组用药剂量参照上述两组联合干预。通过组间对照的方法，对比研究表皮葡萄球菌在生理盐水、RNAⅢ抑制肽、左氧氟沙星及两药联合作用下对Hela细胞的黏附情况。　　结果与结论：空白组 Hela 细胞层表面有大量细菌黏附，而各用药组细菌黏附的数量均显著低于空白组(P<0.001)，左氧组光点计数明显低于RNAⅢ抑制肽组(P<0.05)，而联合组Hela细胞层表面黏附的细菌数量进一步降低(P<0.01)。结果证实，RNAⅢ抑制肽可以有效抑制表皮葡萄球菌对宿主细胞表面的黏附，并且与抗生素有协同作用。