同种异体富血小板血浆可增强糖尿病大鼠合成创面胶原

摘要

背景：研究显示，自体富血小板血浆有助于促进糖尿病创面的愈合，但同种异体来源的富血小板血浆对糖尿病创面的作用尚不明确。目的：以链脲佐菌素诱导的糖尿病大鼠创面模型为观察对象，观察同种异体来源的富血小板血浆对糖尿病创面胶原合成的作用。方法：30只SD大鼠，采用链脲佐菌素诱导形成糖尿病模型鼠，随机分成富血小板血浆组和糖尿病组，每组15只。于大鼠背部，形成面积为1 cm2的全层皮肤缺损创面，分别涂以0.1 mL同种异体富血小板血浆或生理盐水，3M贴膜封闭，胶布固定。分别于造模后3，7，14 d时每组各取5只动物观察创面愈合率，并切取创面组织行Masson染色观察胶原分布形态、检测创面组织中羟脯氨酸含量及Ⅰ型、Ⅲ型胶原的mRNA相对表达量。结果与结论：造模后第3，7，14天时富血小板血浆组的创面愈合率均高于糖尿病组(P<0.05)。Masson 染色结果显示富血小板血浆组的创面胶原形成较快、且纤维更粗壮、排列更致密。羟脯氨酸检测结果显示，富血小板血浆组的创面中羟脯氨酸含量明显高于糖尿病组(P <0.01)。RT-PCR检测结果显示术后第3、7、14天时，富血小板血浆组创面内Ⅰ型和Ⅲ型胶原的mRNA表达量均高于糖尿病组(P<0.05)。同时Ⅰ/Ⅲ型胶原比值也较糖尿病组明显增高(P<0.05)。结果表明同种异体来源的富血小板血浆可以有效促进糖尿病创面的愈合，这可能与其增强创面胶原合成的作用有关。%BACKGROUND:Platelet-rich plasma has benefit in diabetic wound healing;however, the effect of al ogeneic platelet-rich plasma is stil unclear. OBJECTIVE:To observe the effect of al ogeneic platelet-rich plasma on col agen synthesis during diabetic wound healing. METHODS:Streptozocin-induced diabetic rats were randomly divided into al ogeneic platelet-rich plasma group and saline control group. Each group had 15 rats. A 1 cm2 ful-thickness skin defect on the rat dorsum was excised. Platelet-rich plasma or saline was applied. Platelet-rich plasma was prepared from the whole blood of al ogeneic healthy rats. Animals of each group were sacrificed on 3, 7, 14 days post operation. The differences of the wound closure rate, morphological character, hydroxyproline content and the relative mRNA expression of the col agen were observed. RESULTS AND CONCLUSION:The wound closure rates were lower in control group on day 3, 7, 14 post operation (P<0.05). Masson staining showed a decreased, disorder, loose col agen fibers distribution in diabetic control wound tissue. The results of the hydroxyproline test showed hydroxyproline content was significantly higher in platelet-rich plasma group at each time point (P<0.01). The relative mRNA expression of type I and III col agen presented a higher expression in platelet-rich plasma treated wound tissue at each time point (P<0.05). And the ratio between type I and III col agen was higher in platelet-rich plasma group at each time point (P<0.05). In summary, al ogeneic platelet-rich plasma can promote diabetic wounds healing, which may attribute to an enhanced col agen synthesis.