BACKGROUND:There is a certain relationship between bone marrow mesenchymal stem cels and articular cartilage, and its celular functional status is one of the causes of osteoarthritis. OBJECTIVE:To explore the correlation between functional status of bone marrow mesenchymal stem cels and disease progression in patients with osteoarthritis. METHODS:The clinical data of 45 patients were colected, including 15 cases undergoing arthroscopic knee cruciate ligament reconstruction (control group), 15 cases of mild osteoarthritis (mild osteoarthritis group), 15 cases of severe osteoarthritis (severe osteoarthritis group). Bone marrow mesenchymal stem cels from three groups were culturedin vitro to observe the morphological changes. Proliferative and differentiation ability was detected and compared between the three groups. RESULTS AND CONCLUSION:At 24 hours of primary culture, the bone marrow mesenchymal stem cels were arranged in regular pattern and spindle-shaped in the three groups with no morphological differences. At 14 days of chondrogenic induction, toluidine blue staining was positive for bone marrow mesenchymal stem cels, but there was stil no difference in the chondrogenic ability in the three groups. Cel counting kit-8 results showed that the proliferative ability of cels was strongest in the control group and weakest in the severe osteoarthritis group. These findings indicate that there is a certain correlation between the functional status of bone marrow mesenchymal stem cels and the progress of the disease.%背景：研究表明骨髓间充质干细胞功能状态的改变是骨关节炎的病因之一。 目的：探讨骨关节炎患者骨髓间充质干细胞功能状态与病情进展的相关性。 方法：收集45例患者的临床资料，其中15例为关节镜下进行膝关节交叉韧带重建患者(对照组)，15例为轻度骨关节炎患者(轻度骨关节炎组)，15例为重度骨关节炎患者(重度骨关节炎组)。体外培养获得3组患者骨髓间充质干细胞，观察其形态变化，检测其增殖能力及软骨向诱导分化能力。 结果与结论：原代骨髓间充质干细胞经24 h培养后呈整齐排列状，且为长梭形，3组骨髓间充质干细胞形态基本一致，不存在显著差异。骨髓间充质干细胞成软骨诱导14 d后行甲苯胺蓝染色鉴定，爬片可见甲苯胺蓝异染颗粒，3组成软骨诱导分化能力无明显差异。CCK-8法检测各组患者骨髓间充质干细胞的增殖能力，其中以对照组增殖能力最强，重度骨关节炎组增殖能力最弱，结果表明骨关节炎患者骨髓间充质干细胞功能状态与病情进展之间存在一定的相关性。
展开▼
