整合素α5β1在膝骨关节炎不同退变程度关节软骨中的差异性表达

摘要

BACKGROUND: Integrin α5β1 has been shown to be related to acetabular cartilage degeneration due to developmental dysplasia of the hip.OBJECTIVE: To investigate the expression of integrin α5β1 in differently degenerated cartilage tissues of the knee after knee osteoarthritis.METHODS: Seventy-five degenerated articular cartilage specimens at the weight-bearing surface of femoral lateral and medial condyles were removed from patients with knee osteoarthritis who underwent total knee replacement, and were then allotted to four groups based on Mankin's grading system: normal (n=6), mild degeneration (n=24), moderate degeneration (n=26) and severe degeneration (n=19) groups. The expression of integrin α5β1 in each group was detected using real-time PCR, immunohistochemistry and western blot assay.RESULTS AND CONCLUSION: The detected results were consistent in the three kinds of assays, and the expression level of integrin α5β1 was ranked as follows: severe degeneration group > moderate degeneration group > mild degeneration group > normal group (P < 0.05). To conclude, differential expression levels of integrin α5β1 are found in different degenerative degrees of articular cartilage in knee osteoarthritis. Furthermore, the expression level of integrin α5β1 is increased with the degenerative severity.%背景:有研究提示整合素α5β1可能与发育性髋关节发育不良髋臼软骨的退变相关.目的:观察整合素α5β1在膝骨关节炎不同退变程度关节软骨中的表达情况.方法:选取全膝关节置换患者股骨内外髁负重面不同退变程度关节软骨(75个标本),结合Bobinac分期,将软骨标本分为4组,正常组(6个标本)、轻度退变组(24个标本)、中度退变组(26个标本)、重度退变组(19个标本).采用Real-time PCR、免疫组织化学及Western blot分别检测整合素α5β1在4组标本中的表达情况.结果与结论:①各种检测方法结果一致,整合素α5β1在4组标本中均有表达,正常组呈低表达,轻、中度退变组表达逐渐增强,重度退变组表达较前3组明显增强,组间差异有显著性意义(P<0.05);②结果说明,整合素α5β1在膝骨关节炎不同退变程度关节软骨中存在差异性表达;关节软骨退变越重,整合素α5β1在软骨中的表达越强.