背景:随着对间充质干细胞基础研究和临床研究的不断深入,其应用范围越来越广.但在间充质干细胞的临床应用中,缺乏暂时储存条件及回输时间的相关研究报道.目的:探索脐带间充质干细胞制备完成后暂存时间对于细胞质量和细胞活性的影响.方法:取冷冻复苏的脐带间充质干细胞和未冷冻的脐带间充质干细胞,放置0-4 ℃储存0,6,12,24 h后分别检测活细胞率、细胞倍增时间、细胞克隆形成率.结果与结论:①未经过冻存的脐带间充质干细胞能在0-4 ℃条件下储存时间较长,储存12 h后细胞开始死亡,细胞活力开始下降;②冷冻复苏的脐带间充质干细胞在0-4 ℃储存时间较短,储存6 h后细胞即开始死亡,细胞活力减弱;③结果表明,脐带间充质干细胞制备完成后应保证在6 h或12 h之内回输,以确保最佳的治疗效果.如果运输距离较长,应优先选用未冻存的间充质干细胞.%BACKGROUND: With the in-depth basic research and clinical research on mesenchymal stem cells, mesenchymal stem cells have wide clinical prospects. However, little is reported on the temporary storage conditions and refusion time of mesenchymal stem cells. OBJECTIVE: To explore the effect of cold storage time on the cell mass and viability of umbilical cord mesenchymal stem cells.METHODS: Frozen-thawed and unfrozen umbilical cord mesenchymal stem cells were prepared and stored at 0-4 ℃. The cell viable cell rate, cell doubling time and colony forming efficiency were detected after 0, 6, 12, and 24 hours. RESULTS AND CONCLUSION: Unfrozen cells could maintain the cell biological activity at 0-4 ℃until dead cells appeared with the presence of decreased cell viability at 12 hours after storage. Frozen-thawed cells were unable to be stored at 0-4 ℃ for a long time, and cells began to die and the cell viability was weakened at 6 hours after storage. These findings indicate that umbilical cord mesenchymal stem cells should be injected into patients within 6 or 12 hours after preparation, to ensure the best therapeutic effects. If there is a longer transport distance, it is preferred to use unfrozen mesenchymal stem cells.
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