利胆消黄汤对梗阻性黄疸兔肝细胞线粒体氧化损伤的保护作用

摘要

Objective To study the protective effect of Gallbladder eliminating jaundice soup for rabbit liv⁃er mitochondrial oxidative damaged by obstructive jaundice. Method Thirty New Zealand rabbits were divid⁃ed into the control group, the obstructive jaundice group and the traditional Chinese medicine group randomly. The rabbit obstructive jaundice models were established by bundling up middle of the biliary tract. The tradition⁃al Chinese medicine group rabbits were treated with 50 mL gallbladder eliminating jaundice soup once a day by gavages after being modeled for 7 days. According to 14-day time points after being modeled,liver tissue in all groups was taken and the index-8-OhdG and MDA were detected by ELISA method and TBA. Enzyme colorime⁃try and at the same time drawing arterial blood to detect the contents of AST and TBIL to evaluate the liver func⁃tion. Result The levels of 8-OhdG and MDA(1489 ± 262) pg/mgprot,(0.82 ± 0.21) nmol/mgprot in the liver tissuse of the obstructive jaundice group were higher than those(210 ± 78) pg/mgprot,(0.21 ± 0.09) nmol/mgprot in the control group(P<0.05). The levels of those(249±212)pg/mgprot,(0.23±0.17)nmol/mgprot in the tradi⁃tional Chinese medicine group were lower than those in the obstructive jaundice group with statistically signifi⁃cant differences(P<0.05). The ASTand TBIL levels(102.3±32.6)IU/L,(16.3±5.1)μmol/L in the obstructive jaundice group were higher than those(49.5±9.8)IU/L,(5.4±1.1)μmol/L)in the control group(P<0.05). Com⁃paring with the obstructive jaundice group, the levels of AST and TBIL(52.9±12.4)IU/L,(5.6±1.3)μmol/L in the traditional Chinese medicine group were lower than those with statistically significant differences(P<0.05). Conclusion The gallbladder eliminating jaundice soup shows a repairing effect on the oxidative dam⁃age of rabbit liver mitochondria about obstructive jaundice, the mechanism may be related to inhibiting the formation of 8-OHdG and MDA in the liver mitochondria.%目的：研究利胆消黄汤对梗阻性黄疸兔肝细胞线粒体氧化损伤的保护作用。方法：新西兰白兔30只，随机分为对照组、梗阻性黄疸组和中药组，采用胆总管中段捆扎法制备兔梗阻性黄疸模型；中药组于造模后第7d开始给予利胆消黄汤灌胃，50 mL/次，1次/d。于造模后第14 d取各组兔肝组织，采用ELISA法和硫代巴比妥酸(TBA)酶比色法分别对肝组织内的8-羟基-2-脱氧鸟苷酸（8-OHdG）和丙二醛（MDA）含量进行检测；并同时取动脉血，检测血清谷草转氨酶（AST）活性和总胆红素（TBIL）含量。结果：梗阻性黄疸组肝组织8-OhdG和MDA含量分别为(1489±262) pg/mgprot、(0.82±0.21) nmol/mgprot，对照组二者的含量分别为(210±78) pg/mgprot、(0.21±0.09) nmol/mgprot，梗阻性黄疸组中8-OhdG和MDA含量高于对照组（P＜0.05）。中药组肝组织8-OhdG和MDA含量分别为(249±212) pg/mgprot、(0.23±0.17) nmol/mgprot，较梗阻性黄疸组明显降低（P＜0.05）。梗阻性黄疸组血清AST和TBIL含量分别为(102.3±32.6) IU/L、(16.3±5.1)μmol/L，较对照组二者的含量[（49.5±9.8）IU/L，（5.4±1.1）μmol/L]升高（P＜0.05）。与梗阻性黄疸组相比，中药组血清AST含量（52.9±12.4）IU/L和TBIL含量（5.6±1.3）μmol/L降低有显著性差异（P＜0.05）。结论：利胆消黄汤对兔肝细胞线粒体内氧化应激所导致的损伤具有修复作用，其机制可能与抑制肝细胞线粒体8-OHdG和MDA生成有关。