DJ-1、张力蛋白同源10q丢失的磷酸酶基因和黏着斑激酶在口腔癌及癌前病变中的表达

摘要

目的 探讨在口腔鳞状细胞癌及癌前病变中DJ-1、张力蛋白同源10q丢失的磷酸酶基因(phophatase and tensin homolog deleted on chromosome 10,PTEN)和黏着斑激酶(focal adhesion kinase,FAK)的作用,以期为口腔癌的早期防治及基因靶向治疗提供依据.方法 收集广东省口腔医院黏膜牙周科2005年1月至2010年12月的病理标本,包括口腔鳞状细胞癌44例(口腔鳞状细胞癌组)、单纯增生20例(单纯增生组)、异常增生15例(异常增生组)和健康对照标本10例(健康对照组).采用免疫组化方法检测D J-1、PTEN及FAK 3种蛋白的表达.结果 免疫组化结果显示DJ-1在健康对照组、单纯增生组、异常增生组及口腔鳞状细胞癌组中的表达分别为3/10、50％ (10/20)、10/15和91％ (40/44),口腔鳞状细胞癌组织中DJ-1的表达显著增高,与其他组相比差异有统计学意义(P＜0.05)；PTEN在健康对照、单纯增生组的阳性表达均为100％,在异常增生及口腔鳞状细胞癌组中的表达分别为13/15与45％(20/44),而FAK在健康对照、单纯增生、异常增生及口腔鳞状细胞癌组中的表达分别为10/10、95％ (19/20)、14/15和80％(35/44),表明PTEN和FAK在OSCC组织中的表达降低,与其他组相比差异均有统计学意义(P＜0.05)；相关性分析表明D J-1与PTEN之间呈负相关关系(r=-0.75,P＜0.05),PTEN和FAK间呈正相关关系(r=0.28,P＜0.05).结论 D J-1、PTEN、FAK在口腔鳞状细胞癌的发生过程中可能起重要作用.%Objective To investigate the expression of DJ-1,phophatase and tensin homolog deleted on chromosome 10 (PTEN) and focal adhesion(FAK) in patients with oral squamous cell carcinoma (OSCC) and precancerous lesions.Methods Immunohistochemical staining (SP method) was used to detect the expression of DJ-1 and PTEN in the biopsy mucosa tissues from 44 cases of oral squamous cell carcinoma,20 cases of hyperplasia of oral mucosa,15 cases of dysplasia of oral mucosa and 10 cases of normal mucosa.Results The positive rate of DJ-1 in OSCC was 91％ (40/44),which was significandy higher than that in other non-malignant groups including hyperplasia(50％),dysplasia (67％)and normal mucosa(30％) (P ＜ 0.05).The positive rates of PTEN was 45％ (20/44) in OSCC,which were significantly lower than that in other non-malignant groups (P ＜ 0.05) including hyperplasia (100％),dysplasia (87％)and normal mucosa(100％) (P ＜0.05).The positive rates of FAK was 80％ (35/44) in OSCC,which were significantly lower than that in other non-malignant groups (P ＜ 0.05) including hyperplasia(100％),dysplasia(95％) and normal mucosa(100％) (P ＜ 0.05).In all of these tissues,a negative correlation was found between the expression of DJ-1 and PTEN (r =-0.75,P ＜ 0.05),while a positive correlation was found between the expression of DJ-1 and PTEN (r =0.28,P ＜ 0.05).Conclusions DJ-1,PTEN and FAK may play an important role in the pathogenesis of OSCC.