针刺对高脂血症小鼠血脂的影响及其机制研究

摘要

目的：观察针刺对高脂血症小鼠血脂的影响，探讨针刺逆转内皮功能障碍的机制。方法40只ApoE(-/-)小鼠随机分为对照组、针刺非穴位组、针刺穴位组和他汀类药物组，分别针刺穴位、非穴位以及辛伐他汀灌胃。8周后测量各组小鼠总胆固醇(TC)。采用ELISA法检测血浆中血管紧张素Ⅱ(AngⅡ)、内皮素-1(ET-1)及一氧化氮(NO)的含量。采用Western blotting法检测小鼠心脏AngⅡ1型受体(AT1R)、内皮素-1A受体(ETAR)的蛋白含量。结果针刺穴位组和他汀类药物组与对照组相比，TC含量降低，血浆AngⅡ、ET-1含量降低，NO含量升高，心脏组织中AT1R、ETAR蛋白含量降低(P<0.05)。结论针刺内关和丰隆穴可以抑制ApoE(-/-)小鼠血脂升高，降低外周血AngⅡ、ET-1水平，升高外周血NO含量，抑制心脏组织中AT1R、ETAR。%Objective To observe the effects of acupuncture on blood lipids of hyperlipidemia mice, and explore the mechanism of acu-puncture against endothelial dysfunction. Methods 40 ApoE (-/-) mice were randomly divided into control group (C), acupuncture at non-acupoint group (D), acupuncture at acupoint group (E), and simvastatin group (F) with 10 cases in each group. After 8 weeks, total cho-lesterol (TC), the plasma angiotensin II (Ang II), endothelin-1 (ET-1) and nitric oxide (NO) were measured with enzyme-linked immuno sor-bent assay (ELISA). The mice myocardial angiotensin II type 1 receptor (AT1R), and endothelin-1 type A receptor (ETAR) protein were de-tected with Western blotting. Results The blood lipid, the content of plasma Ang II and ET-1, and the level of AT1R and ETAR in the heart tissue were significantly lower, and the content of NO was significantly higher in groups E and F than in group C (P<0.05). Conclusion Acu-puncture can inhibit the level of blood lipids in ApoE(-/-) mice, reduce the levels of Ang II and ET-1, increase the level of NO in peripheral blood, and inhibit the expression of AT1R, ETAR in heart tissue.