电针结合超短波疗法对大鼠全横断脊髓损伤后GAP-43和Caspase-3表达的影响术

摘要

Objective:To observe the effects of electroacupuncture combined with ultrashort wave therapy on changing the expression of nerve growth related protein (GAP-43) and neural cell apoptosis related protein (Caspase-3) in the injured spinal cord of the rat,and study the subsequent primary recovery mechanism of the spinal cord after transection.Method:To moulding the rat with spinal cord injury (SCI),75 rats were randomly divided into 5 groups:sham operation group,SCI group,SCI + electroacupuncture treatment group (electroacupuncture group),SCI + ultrashort wave therapy group (ultrashort wave group),SCI + electroacupuncture treatment + ultrashort wave therapy group (combination group),each group had 15 rats.Detected the expression of GAP-43 and Caspase-3 at 1w,2w,3w,4w,5w after moulding.BBB score of rats in every group were taken at the same time points.Result:The BBB scores increased (P＜0.05) in every treatment group at every time points.Comparing with the SCI group,the expression of GAP-43 significantly increased in the electroacupuncture group and the expression of Caspase-3 significantly decreased in the electroacupuncture group,ultrashort wave therapy group and combination treatment group (P＜0.05).Furthermore,the expression of GAP-43 significantly increased and the expression of Caspase-3 significantly decreased in the combination treatment group compared with SCI group,electroacupuncture group and ultrashort wave therapy group at lw,2w,3w,4w,5w time points(P＜ 0.05).Conclusion:①Electroacupuncture treatment and ultrashort wave therapy can increase the expression of GAP-43 and decrease the increased expression of Caspase-3 in the transection area of the rat spinal cord.②Electroacupuncture treatment combined with ultrashort wave therapy is more effective for the recovery of motor function of the rat after spinal cord transection.%目的:观察电针结合超短波疗法对脊髓全横断损伤大鼠损伤部位神经生长相关蛋白(GAP-43)、神经细胞凋亡相关蛋白(Caspase-3)的影响.探讨电针结合超短波疗法对脊髓全横断损伤大鼠运动功能恢复的机制.方法:复制并评价SCI大鼠模型.将75只大鼠随机分为5组:假手术组、脊髓损伤组(SCI组)、SCI+电针治疗组(电针组)、SCI+超短波治疗组(超短波组)、SCI+电针治疗组+超短波治疗组(联合组),每组15只大鼠.检测各组1周、2周、3周、4周、5周5个时间点GAP-43、Caspase-3的表达,对各组大鼠进行BBB评分.结果:在不同时间点各治疗组的脊髓损伤大鼠BBB评分均有所提高(P＜0.05).与SCI组相比,电针组、超短波组和联合组GAP-43的表达显著增加,Caspase-3的表达显著减少(P＜0.05).联合组在术后第1周、2周、3周、4周、5周较其他组GAP-43的表达显著增加,Caspase-3的表达显著减少(P＜0.05).结论:①电针疗法和超短波疗法都可以促进大鼠损伤区脊髓组织内GAP-43表达增多和抑制大鼠损伤区脊髓组织内Caspase-3表达增多.②联合治疗对大鼠运动功能的恢复更为有效.