督脉电针结合超短波疗法对大鼠脊髓全横断损伤后脑源性神经生长因子和神经轴突生长抑制剂-A表达的影响

摘要

Objective:To investigate the mechanism underlying the effects of Du Meridian electroacupuncture combined with ultrashort wave therapy on spinal cord injury (SCI) by detecting the motor function recovery and the expressions of BDNF and Nogo-A in the spinal lesion segments.Method:Copying and evaluating the models of rats with spinal cord complete transections,100 female rats were randomly divided into 5 groups:Sham-control group,the group of SCI,SCI with Du Meridian electroacupuncture (DMEA) group,SCI with ultra-short wave group,SCI with DMEA plus ultrashort wave group (n=20).The BBB scores and expressions of BDNF and Nogo-A in groups at 7d,14d,21d and 28d were tested.Result:Compared with the Sham-control group,the BBB scores increased significantly in all treatment groups.Moreover,the BBB scores of the combination treatment group was significantly higher than that of the ultrashort wave group and DMEA group at 7d,14d,21d and 28d post-operation (P＜0.01).Comparing with the Sham-control group,the expression of BDNF significantly increased and the expression of Nogo-A significantly decreased.Furthermore,the expression of BDNF significantly increased and the expression of Nogo-A significantly decreased in the combination treatment group when compared with the ultra-short wave group and DMEA group at 7d,14d,21d and 28d post-operation (P＜ 0.01).Conclusion:①DMEA combined with ultrashort wave therapy can improve the expression of BDNF after SCI and decrease the expression of Nogo-A to promote neural repair.②The combination therapy is more effective to promote motor function recovery in rats than using them separately.%目的:通过检测大鼠脊髓损伤(SCI)后运动功能恢复情况和脑源性神经生长因子(BDNF)、神经轴突生长抑制剂-A(Nogo-A)的表达来探讨联合应用督脉电针和超短波疗法对SCI的作用机制.方法:复制并评价脊髓全横断损伤大鼠模型,100只雌性大鼠随机分为5组:假手术组、SCI组、SCI+督脉电针组、SCI+超短波组、SCI+督脉电针+超短波组(n=20),检测各组7d、14d、21d、28d这4个时间点BDNF和Nogo-A表达,对各组大鼠进行BBB评分.结果:与模型组相比,各治疗组的BBB评分显著增加,联合组在术后第7d、14d、21d、28d较超短波组和督电组BBB评分显著增加,具有显著性差异(P＜ 0.01).与模型组相比,各治疗组BDNF的表达均显著增加,Nogo-A的表达均显著减少,联合组在术后第7d、14d、21d、28d较超短波组和督电组BDNF的表达显著增加,Nogo-A的表达显著减少,具有显著性差异(P＜ 0.01).结论:①联合应用督脉电针与超短波治疗,能够提高SCI后BDNF的表达及抑制Nogo-A的表达来促进神经的修复.②联合疗法对大鼠运动功能恢复更佳.