宠物源大肠杆菌的血清型和毒力基因及耐药性调查

摘要

为研究宠物源致病性大肠杆菌(Escherichia coli)的血清型、毒力基因和耐药性之间的相关性,本研究由健康和患病犬、猫直肠拭子样品中分离177株E.coli,并采用玻片凝集法鉴定其血清型,结果显示分离株中定型菌株135株,分别属于20个不同的血清型,其中O1、O2和O8为主要的流行血清型.PCR方法检测11种毒力相关基因,并采用琼脂稀释法测定分离菌株对14种抗菌药的敏感性,结果表明3个血清型的菌株拥有相似的耐药表型,但毒力基因谱不同.62％的分离菌株携带fimH基因,并且毒力基因组合iroN+ yF、iroN+ fimH和traT+sitA比较流行.55％的O1血清型携带fimH基因,并且多数耐受四环素、多西环素、头孢噻吩和庆大霉素；20.8％的O2血清型菌株携带traT和sitA基因；50％的O8血清型携带traT和fimH基因.3个血清型分离菌株多数对安普霉素和阿米卡星敏感.%To investigate the E. Coli isolated from pets in Guangzhou, the islates were primarily test by agglutination with specific O serogroup antisera. Twenty O serogroups were identified for 135 E. Coli isolates, of which O1, O2 and O8 were dominant serogroups. Virulence genes detection by PCR method showed that 62% of the isolates possessed fimH gene and most of the E. Coli isolates with a combinations of iroN+hlyF, iroN+fimH and traT+sitA. Furthmore, 55% of O1 serogroup carried fimH gene and most of O1 isolates were resistant to tetracycline, doxycycline, ceftiofur and gentamycinl. While 20.8% of O2 isolates possessed traT and sitA genes and were resistant to tetracycline and doxycycline. Fifty percent of O8 serogroup strains possessed traT and fimH genes, and most of the 3 O serogroups were susceptible to apramycin and amikacin.