邢台地区致鸡卵黄性腹膜炎大肠杆菌血清型鉴定、毒力基因检测及耐药性分析

摘要

为分析邢台地区致鸡卵黄性腹膜炎大肠杆菌(E.coli)分离株的血清型、毒力基因和耐药性,本实验采用常规鉴定方法和16S rRNA PCR的方法,从邢台地区不同养鸡场46份患卵黄性腹膜炎的40周龄～45周龄蛋鸡病料组织中分离鉴定出37株E.coli.人工感染1日龄的雏鸡,结果显示32株分离菌为致病性E.coli,采用玻板凝集法检测其血清型分布,结果显示定型的27株致病性E.coli分离株共属于11个血清型,以O78、O148、O2及O8为优势血清型.采用PCR方法检测32株致病性E.coli分离株14种毒力基因,其中rp、fyuA、ompT、Issa、iucDa、iutA、iroN、hlyF、fimC毒力基因检出率最高,分别为100％、96.9％、93.8％、86.7％、84.3％、71.9％、65.7％、56.3％和50％,其余毒力基因检出率较低.耐药性分析结果显示,32株致病性E.coli分离株仅对头孢克肟、左氧氟沙星、阿奇霉素、头孢曲松和氟苯尼考5种药物高度敏感,对其他药物存在不同程度的耐药性.本研究为防治致病性E.coli引起蛋鸡卵黄性腹膜炎提供了实验依据.%To investigate the serotype,virulence genes and drug resistance of the E.coli isolates causing chicken salpingitis and peritonitis in Xingtai area,a total of 37 E.coli were isolated from 46 salpingitis and peritonitis samples of 40-45 weeks old chicken in different chicken farms in Xingtai area,and identified by bacteria characteristics and 16S rRNA PCR methods.The results of the artificial infection in 1 days old chickens showed that 32 isolates were pathogenic E.coli;The serotype of 32 isolates were determined by direct agglutination test with specific antisera,27 pathogenic E.coli isolates belonged to 11 kinds of serotypes,of which O78,O148,O2 and O8 were the predominant serotypes.The positive rates of the virulent genes ofirp2,fyuA,ompT,Issa,iucDa,iutA,iroN,hlyF and fimC genes were 100％,96.9％,93.8％,86.7％,84.3％,71.9％,65.7％,56.3％ and 50％,respectively,in the 32 E.coli isolates by PCR method.Assay of drug resistance showed the 32 isolates were highly sensitive to 5 kinds of drugs,such as levofloxacin,azithromycin,ceftriaxone,florfenicol.The sensibility to other drugs was low.These data provide the basis for the prevention and treatment of the E.coli causing chicken salpingitis and peritonitis.