为分析2015年～2016年广东省猪流行性腹泻病毒(PEDV)流行株的遗传变异情况,本研究对16株广东地区的PEDV流行株部分S1基因进行克隆和测序,并将测序结果与GenBank中PEDV参考株S1基因进行同源性分析并构建系统进化树.结果显示,16株PEDV流行株核苷酸序列同源性为93.8％～100.0％,与国内参考株核苷酸序列同源性为70.7 ％～98.1％.PEDV S1基因遗传进化分析显示,16株流行株与国内大部分流行株亲缘关系较近,与疫苗株CV777、中国早期分离株CH/S、韩国流行株DR13、泰国流行株TH/AY/2.7/ 12、日本株14JM/01、美国株Minnesota/XW001等亲缘关系较远.此外,16株广东PEDV流行株与华南地区2014年之前分离株CH/GDQY/2011、CH9/FJ、CH/GDGZ/2012、FM/YN/2013等属群不同,亲缘关系较远.试验结果表明广东省当前流行的PEDV存在基因变异,并已经成为优势病毒株.%In order to analyze the genetic variation of epidemic diarrhea virus (PEDV) in Guangdong province from 2015 to 2016,partital S1 genes of 16 PEDV epidemic strains isolated from Guangdong region were cloned and sequenced.The results of sequence alignment and analysis showed that the nucleotide sequence homologies were 93.8％-100.0％ between the 16 PEDV isolates,and were 70.7％-98.1％ among the 16 PEDV isolates and the domestic reference strains.The results of genetic evolution analysis of PEDV S1 gene showed the 16 isolates had close phylogenetic relationship with most of the epidemic strains in China,while they were far from relationship with vaccine strains CV777,early isolates CH/S of China,epidemic strains DR13 of South Korea,epidemic strain TH/AY/2.7/12 of Thailand,14JM/01 strain of Japan,and Minnesota/XW001 strain of USA.The 16 isolates were different from those in Southern China region before 2014,such as CH/GDQY/2011,CH9/FJ,CH/GDGZ/2012,FM/YN/2013.The results reveal that the current prevalent PEDV in Guangdong exists gene variation and has become the dominant strain.
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