Objective To observe the toxicity of arctigenin in Beagle dogs by continuous subcutaneous injection,so as to provide reference information for the clinical research.Methods 40 healthy conventional Beagle dogs,half male and half female,were divided into 5 groups:control group,polyethylene glycol (PEG) control group and arctigenin (NBZGY) 6,20,60 mg·kg-1 groups,8 dogs in each group.Dogs of each group were given 1.2 mL·kg1 by subcutaneous injection 1 time per day for 28 days,and observed for 2 weeks after the last administration.During the experiment,the general state of animals,food consumption,body weight,hematology,blood biochemistry parameters were detected.Results The stimulus response caused by PEG had been seen in the administration location of PEG-control group and NBZGY each dose group,60 mg·kg-1 NBZGY group could strengthen the stimulation,after the last administration,the stimulus response could be partly recovered.20 and 60 mg·kg-1 induced injury of liver and biliary duct in contrast to the control group during the period of administration.At the end of convalescence,dogs in 20 mg·kg-1 group showed a recovery of the injury of liver and biliary duct,but the injury of liver and the biliary duct had not recovered obviously in the 60 mg·kg-1group.There was no overt toxicity in 60 mg·kg-1 group except the stimulus response in the administration location.Conclusion Repeated subcutaneous injection of large dose of NBZGY can induce injury of liver and biliary duct and strengthen the reaction to the stimulus caused by PEG in dogs.%目的 观察连续皮下注射牛蒡子苷元(NBZGY)对Beagle犬产生的毒性反应,为临床用药安全提供参考.方法 普通级Beagle犬40只,雌雄各半,分为5组:对照组、聚乙二醇(PEG)对照组及NBZGY 6、20、60 mg· kg-1组,每组8只.各组犬按1.2 mL· kg-1的给药体积经皮下注射给药,每天1次,连续给药28天,停药后观察2周.实验期间对动物的一般状况、摄食量、体重、血液学、血液生化等指标进行了检测和检查.结果 PEG对照组及NBZGY各剂量组犬给药部位可见与PEG相关的刺激性反应,60 mg· kg-1可增强该刺激性,停药后给药部位刺激性可见部分恢复.给药期间,20、60 mg·kg-1导致犬出现肝损伤和胆管损伤.恢复期结束,20 mg· kg-1组犬肝胆损害恢复正常,60 mg· kg-1组犬肝胆损伤未见明显恢复.除注射部位刺激性外,6 mg·kg 1组未见其他明显毒性.结论 Beagle犬连续皮下注射大剂量的NBZGY,可增强Beagle犬对PEG相关刺激的反应.并引起犬的肝胆损伤.
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