骨髓间质细胞和非骨髓间质细胞源细胞移植后机体免疫反应差异的比较

摘要

AIM: To analyze the characteristics of immune response in the animals after transplantation of bone marrow stromal cells ( BMSCs ) or non - BMSCs - derived cells.METHODS: Rat BMSCs and Buffalo rat liver ( BRL ) cells were transplanted into SD rats and New Zealand rabbits by intravenous and subcutaneous injections.The differences of immune response in the animals received these two types of cells were evaluated by comparative analysis of the lymphocytes and monocytes in peripheral blood as well as the histopathological characteristics of the subcutaneous transplantation sites.RESULTS: The increase intensity of the lymphocyte number in BMSCs group was lower than that in BRL group, regardless of allograft or xenograft.The immune reaction of the animals to BMSCs transplantation was a little slower.It was obviously dissimilar that the increase intensity of monocyte number in BMSCs group was higher than that in BRL group.Pathological cutaneous sections showed that neither obvious necrosis nor inflammatory cells was observed in the vicinity of BMSCs transplantation sites.CONCLUSION: The intensity of immune response induced by BMSCs is lower than that by BRL cells.The anti - rejection ability of BMSCs in cell transplantation is better than that of BRL cells.%目的:比较骨髓间质细胞(BMSCs)和非骨髓间质细胞源细胞移植后体内的免疫反应特征.方法:选用SD大鼠和新西兰大白兔作为细胞移植的受体动物,分别将大鼠BMSCs和肝细胞株BRL经静脉和皮下植入动物体内,通过分析外周血淋巴细胞和单核细胞数目变化及皮下移植位点组织病理学特征,揭示2种细胞移植后体内的免疫反应差异.结果:无论在同种异体移植组或异种交叉移植组,BMSCs所引起的淋巴细胞增殖强度均低于BRL移植组,动物对BMSCs移植的免疫反应较为迟钝;而单核细胞的增殖能力则是BMSCs移植组高于BRL移植组.组织病理学结果显示,BMSCs移植组未在移植位点附近观察到明显的细胞坏死及炎症细胞浸润.结论:由BMSCs引发的免疫反应强度低于BRL,在细胞移植中抵御免疫排斥的能力要优于BRL.