首页> 外国专利> METHOD FOR CULTURING MYCOPLASMA CONTAMINATION-FREE CELLS AND METHOD FOR REMOVING MYCOPLASMA CONTAMINATION OF CELLS

METHOD FOR CULTURING MYCOPLASMA CONTAMINATION-FREE CELLS AND METHOD FOR REMOVING MYCOPLASMA CONTAMINATION OF CELLS

机译:培养无骨髓间质污染细胞的方法和去除细胞间质污染的方法

摘要

Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.
机译:提供了一种用于培养细胞的方法,该方法通过使用通过培养具有两性甾醇生产率的菌株,优选菌株如和属于鞭毛藻的类似物,或使用从提取物中获得的馏分;以及通过使用两性酚衍生物培养被支原体污染的细胞来去除细胞的支原体污染的方法。另外,提供了一种去除细胞的支原体污染的方法,该方法包括单细胞分离酶溶液处理和细胞聚集体去除的组合。

著录项

  • 公开/公告号US2012244614A1

    专利类型

  • 公开/公告日2012-09-27

    原文格式PDF

  • 申请/专利权人 JAE-JIN HAN;

    申请/专利号US201013513743

  • 发明设计人 JAE-JIN HAN;

    申请日2010-12-08

  • 分类号C12N5/07;

  • 国家 US

  • 入库时间 2022-08-21 17:34:12

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号