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METHOD FOR CULTURING MYCOPLASMA CONTAMINATION-FREE CELLS AND METHOD FOR REMOVING MYCOPLASMA CONTAMINATION OF CELLS

机译:培养无骨髓间质污染细胞的方法和去除细胞间质污染的方法

摘要

The present invention provides a method for culturing cells, which is able to prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, the present invention provides a method for removing mycoplasma contamination of the cells, comprising a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.
机译:本发明提供了一种细胞培养方法,该方法能够通过使用通过培养具有两性甾醇生产率的菌株,优选菌株如而获得的生物质提取物来培养细胞,从而防止支原体污染的可能性。 ,或属于鞭毛虫的,或软骨粉等,或使用从提取物中获得的级分;以及通过使用两性酚衍生物培养被支原体污染的细胞来去除细胞的支原体污染的方法。另外,本发明提供了一种去除细胞的支原体污染的方法,该方法包括单细胞分离酶溶液处理和细胞聚集体去除的组合。

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