微管相关蛋白2和巢蛋白在人胚胎小肠中的表达

摘要

目的:探讨微管相关蛋白2(MAP-2)和巢蛋白(nestin)与人胚胎小肠壁组织发育的关系.方法:应用免疫组织化学PV法和图像分析(NIS-DR)软件检测第2、3、4个月龄段,人胚胎小肠组织内MAP-2和nestin的表达分布规律,数据的组间比较采用单因素方差分析.结果:第2、3、4个月龄段,MAP-2主要表达于人胚胎小肠壁黏膜下和肌间神经丛内神经细胞和纤维,随胎龄的增大,肌间神经丛内神经细胞的阳性强度增强,两两比较,差异有统计学意义(P<0.05).Nestin蛋白在小肠壁各层均有阳性表达,其黏膜下层和肌间神经丛内阳性细胞数量随胎龄增大而呈先增高再降低的趋势,两两比较,差异有统计学意义(P<0.05).结论:MAP-2和nestin参与调节人胚胎小肠壁组织的生长发育过程.%AIM; To explore lhe relationship belween expression of microlubule - associated prolein 2 (MAP- 2)/neslin and developmenl of small inlesline in human embryos. METHODS; The expression of MAP -2 and neslin proleins was delecled in lhe second, ihird and fourth monlhs of felal human embryonic small inleslinal lissues by lhe PV melhod of immunohislochemislry. The posilive cell counls and posilive inlensilies were analyzed wilh Nikon imaging syslem (NIS -DR). One-way ANOVA was applied Lo compare lhese posilive cell counls and posilive inlensilies. RESULTS; Al lhe second, lhe ihird and lhe fourth monlh of human embryos, MAP -2 posilive expression was observed in lhe neural cells and neural fibers of lhe inlermuscular nerve plexus and submucosal plexus in lhe developing small inleslines. Wilh lhe felal age increasing, lhe expressive inlensily of MAP -2 posilive cells in lhe neural lissues of lhe inlermuscular nerve plexus showed an uplrend. The resulls of posilive inlensily values of lhe MAP -2 posilive cells delecled in lhe 3 felal age periods showed significant difference ( P < 0. 05 ) . Neslin posilive expression was seen in all layers of lhe small inlesline, and lhe number of posilive cells was firsl increased and lhen decreased in lhe developing small inleslines of human embryos wilh lhe increasing felal age. One - way ANOVA resulls also showed significant difference (P <0. 05) . CONCLUSION; MAP- 2 and neslin regulale lhe developmenl of small inleslinal lissues in human embryos.