Ikaros的3种亚型对人卵巢癌 SKOV3细胞增殖的影响

摘要

目的：探讨Ikaros的3种亚型对人卵巢癌SKOV3细胞增殖的影响。方法：利用逆转录病毒转染人卵巢癌SKOV3细胞，分别表达Ikaros的3种亚型（ IK1、IK2和IK6）；采用CCK－8法分析表达不同亚型后SKOV3细胞的增殖能力；流式细胞术检测细胞周期的改变；Western blot 检测细胞周期相关蛋白的表达。结果： CCK－8结果显示IK1和IK2能明显抑制SKOV3细胞的增殖；细胞周期结果表明IK1和IK2能诱导SKOV3细胞发生G1期阻滞，IK6则对SKOV3细胞的增殖能力和细胞周期则无明显影响；Western blot 检测结果显示，IK1和IK2明显降低cyclin D1和cyclin D2蛋白的表达水平，同时升高p21蛋白的表达水平。 IK6则对SKOV3细胞的cyclin D1、cyclin D2及p21蛋白表达水平无明显改变。结论：IK1和IK2这2种亚型能明显抑制卵巢癌SKOV3细胞的增殖，其机制可能是由于IK1和IK2能降低细胞周期促进蛋白cyclin D1和cyclin D2的表达及增加细胞周期抑制蛋白p21的表达，从而诱导细胞周期发生G1期阻滞。而IK6亚型对SKOV3细胞增殖能力及细胞周期无明显影响。%AIM:ToinvestigatetheeffectofIkarosisoformsontheproliferationofhumanovariancancerSK-OV3 cells.METHODS:Three isoforms of Ikaros, IK1, IK2 and IK6, were transfected into ovarian cancer SKOV3 cells. CCK-8 assay and cell counting were used to detect the effects of Ikaros isoforms on the proliferation of SKOV 3 cells.The cell cycle was analyzed by flow cytometry .The cell cycle-related proteins were detected by Western blot .RESULTS:IK1 and IK2 expression inhibited SKOV 3 cells proliferation .Flow cytometry analysis indicated that IK 1 and IK2 induced SK-OV3 cell cycle arrest at the G 1 phase.IK6 isoform exerted no obvious effect on the proliferation or cell cycle of SKOV 3 cells.Compared with control EV group , IK1 group and IK2 group showed a dramatic elevation in the expression of the cell cycle inhibitor p21, along with a substantial decrease in the expression of the cell cycle inducers cyclin D 1 and cyclin D2, which did not change in IK 6 group.CONCLUSION:IK1 and IK2 significantly inhibit the proliferation of ovarian cancer SKOV3 cells and induce cell cycle arrest at G 1 phase by regulation of cell cycle-related proteins cyclin D1, cyclin D2 and p21, while IK6 isoform exerts no obvious effect on the proliferation and cell cycle of SKOV 3 cells.