目的 探讨喉鳞状细胞癌(squamous cell carcinoma,SCC)中Endoglin(CD105)、促红细胞生成素产生肝细胞受体A2 (erythropoietin-producing hepatocyte receptor A2,EphA2)及其配体EphrinA1蛋白的表达及意义.方法 采用免疫组化SP法检测76例喉SCC和25例喉癌旁组织(adjacent normal laryngeal tissues)中CD105、EphA2及其配体EphrinA1的表达情况,分析它们与喉SCC患者临床病理因素的关系及对喉SCC预后判断的价值.应用SPSS 17.0统计软件包对数据进行分析,计数资料采用x2检验及Fisher精确检验.结果 喉SCC中以CD105标记的微血管密度(microvessel density,MVD)为10.33±2.29,大于喉癌旁组织(1.20 ±1.04),差异有统计学意义(t=18.732,P＜0.05).CD105标记的MVD的表达与喉SCC的T分级、组织学分级、临床分期、淋巴结转移、是否复发有关(F值分别为5.34、4.79、5.36,t值分别为-2.70、2.25,P值均＜0.05).EphA2、EphrinA1蛋白表达的阳性率在喉SCC分别为78.95％ (60/76)、81.85％ (62/76),大于喉癌旁组织的40.00％(10/25)、44.00％ (11/25),差异有统计学意义(x2值分别为13.41、13.26,P值均＜0.05).EphA2蛋白表达与喉SCC的组织学分级、T分级、临床分期、淋巴结转移、是否复发有关(x2值分别为6.25、14.60、15.11、8.52、5.54,P值均＜0.05).EphrinA1蛋白表达与喉SCC的淋巴结转移、T分级、临床分期、是否复发有关(x2值分别为6.44、12.28、16.78、6.44,P值均＜0.05).CD105、EphA2和EphrinA1蛋白表达两两之间均呈正相关(r值分别为0.72、0.74、0.64,P值均＜0.05).生存分析显示,CD105、EphA2、组织学分级、淋巴结转移、临床分期、有无复发是患者预后的独立影响因素(P值均＜0.05).结论 喉SCC中CD105与EphA2、EphrinA1蛋白的表达两两之间呈正相关,三者均促进喉SCC的发生、恶性发展,提示不良预后,联合检测三者的表达有助于喉SCC的诊断及预后判断.%Objective To investigate the expressions of endoglin (CD105),erythropoietinproducing hepatocyte receptor A2 (EphA2) and its ligand ephrinA1 proteins in laryngeal squamous cell carcinoma (LSCC) and the relationship between their expressions and the clinicopathological factors of LSCC.Methods The expressions of CD105,EphA2 and EphrinA1 proteins were detected with immunohistochemical staining in LSCC in 76 cases and adjacent normal laryngeal tissues (ANLT) (S-P) in 25 cases.SPSS 17.0 software was used to analyze the data.Results The mean microvessel density (MVD) value marked by CD105 staining in LSCC was 10.33 ± 2.29,which was significantly higher than that in ANLT(1.20 ± 1.04,t =18.732,P ＜ 0.05).The CD105-MVD was correlated with T stage,histological grading,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (F value was 5.34,4.79,5.36,t value was-2.70,2.56,all P ＜ 0.05).The positive expression rates of EphA2 and EphrinA1 in LSCC were 78.95％ (60/76),and 81.85％ (62/76),which were respectively significantly higher than 40％ (10/25) for EphA2 expression and 44％ (11/25) for EphrinA1,expression in ANLT (x2 value was 13.41,13.26,both P ＜ 0.05).EphA2 expression was correlated with histological grading,T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (x2 value was 6.25,14.60,15.11,8.52,5.54,all P ＜ 0.05).EphrinA1 expression was correlated with T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC (x2 value was 6.44,12.28,16.78,6.44,all P ＜ 0.05).The expressions of CD105,EphA2 and EphrinA1 were positively correlated with each other r value was 0.72,0.74,0.64,all P ＜ 0.05.Survival analysis indicated that the expressions of CD105 and EphA2,histological grading,lymph node metastasis,clinical stage and recurrence were independent factors for tumor prognosis in LSCC (P ＜ 0.05).Conclusions The expressions of CD105,EphA2 and EphrinA1 protein were positively correlated with each other in LSCC.They may play important roles in the tumorigenesis,malignant progression and poor prognosis of LSCC.
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