目的 探讨通过建立去卵巢大鼠模型,研究杜仲叶调节骨代谢防治骨质疏松.方法 80只200g雌性SD大鼠,随即分为模型对照组、假手术对照组、杜仲叶治疗组(10 g/kg-1·d-1)、雌激素治疗组(50 μg/kg-1·d-1).造模4周后连续治疗16周.结果 模型组大鼠机械力学检查使用显微CT(μCT)分析表明骨密度降低,骨关节面塌陷;组织学检查病理组织切片显示骨小梁减少;生化检查骨型碱性磷酸酶、尿钙尿磷反应骨代谢水平的下降,而采用杜仲叶醇提取物及雌激素治疗后,μCT分析表明骨密度相对增多,骨关节面平整,病理组织切片示骨小梁数量正常,生化检查示骨型碱性磷酸酶、血钙血磷、尿钙尿磷均与正常对照组无明显差异.结论 杜仲叶可以维持骨代谢平衡防治去势大鼠骨质疏松症.相对模型组其主要能增高骨密度、增加骨小梁数量、以及改善骨代谢平衡.%Objective To investigate the effect of eucommia ulmoides oliv leaves on the regulation of bone metabolism for the prevention and treatment of osteoporosis using established ovariectomized rat model. Methods Eighty 200g female SD rats were selected. They were randomly divided into 4 groups: model control group, sham-operation control group, eucommia ulmoides oliv leaves treatment group ( 10 g/kg d-1 ), and estrogen therapy group ( 50 μg/kg-1 ? D-1 ). After successful modeling for 4 weeks, the continuous therapy lasted for 16 weeks. Results In model control group, mechanical inspection using micro CT ( |xCT ) analysis showed decreases in bone mineral density ( BMD ) and collapse of joint surface in rats. Histological examination showed a decrease in the bone trabecular. The biochemical test results such as bone type alkaline phosphatase, urinary calcium, and urinary phosphorus indicated that the level of bone metabolism declined. After the treatment of eucommia ulmoides oliv leaves ethanol extraction and estrogen, μCT analysis showed relative increases in BMD and smooth joint surface. Histopathology showed normal bone trabecular number. Biochemical tests showed that the levels of bone alkaline phosphatase, serum calcium, serum phosphorus, urinary calcium, and urinary phosphorus had no significant difference with those in normal control group. Conclusion Eucommia ulmoides oliv leaves can maintain bone metabolic balance, and prevent and treat osteoporosis in castrated rats. The effect is mainly on the increase of BMD, the increase of bone trabecular number, and the improvement of bone metabolic balance.
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