建立雄性大鼠骨质疏松模型的方法学研究

摘要

Objective To evaluate the methodology feasibility of building osteoporosis model in male rats experimentally using drug induction and surgical castration,in order to provide supports for further study.Methods 40 male SD rats were divided into four groups randomly (10 in each group):the control group,the surgery group,the dose group and the combined intervention group.Surgical method was bilateral testicular resection.Rats in the dose group were injected D-galactose subcutaneously.Rats in the combined intervention group were treated with both methods.We then determined weight,bone mineral density (BMD),bone mineral salt content (BMC),contents of soft tissue,fat and muscle before and 8 weeks after the intervention.Results Eight weeks after the intervention,BMD of the three experimental groups decreased relatively to the controls.BMD of the combined intervention group and surgery group reduced by 12％ and 7％,respectively,with statistical significances (P ＜ 0.01).BMD of the dose group decreased by 2％,which was not statistically significant (P ＞ 0.05).There were no significant differences between the four groups in bone mineral salt content (P ＞ 0.05) and body weight (P ＞ 0.05).However,the combined intervention group had 5.6％ lower BMD than the surgery group (P ＜0.05) and the fat content of the three experimental groups were lower than that of the control group (P ＜ 0.01).Conclusion At the 8th week,the male rat osteoporosis model was established.Using the castration method and the injection of D-galactose at the same time can speed up the establishment of male rat osteoporosis model.After developing osteoporosis,fat content of male rats reduced.%目的 探讨药物诱导与手术去势构建雄性大鼠骨质疏松模型的可行性,为进一步研究男性骨质疏松提供支持.方法 雄性SD大鼠40只,随机分为4组(每组10只):空白对照组、手术组、给药组和共处理组.对照组未做处理;手术组行双侧睾丸切除方法;给药组行皮下注射D-半乳糖;共处理组同时行手术和皮下注射D-半乳糖.于干预前及干预后第8周测定体重,骨密度和骨矿盐含量,组织、脂肪、肌肉含量.结果 术后第8周,三组实验组骨密度较对照组骨密度降低,共处理组和手术组骨密度分别下降12％和7％,统计学差异显著(P＜0.01);给药组骨密度下降2％,不存在统计学意义(P＞0.05);各组骨矿盐含量无差异(P＞0.05).共处理组骨密度较手术组骨密度低5.6％,肌肉含量低19.8％,存在统计学差异(p＜0.05).四组间体重无统计学差异(P＞0.05);三组实验组均低于对照组脂肪含量,统计学差异显著(P＜0.01),三组间脂肪含量无统计学差异(P＞0.05).结论 本研究手术去势法8周可以建立骨质疏松模型;去势法和皮下注射D-半乳糖联合使用8周建立骨质疏松模型骨密度下降更为明显;雄性动物骨质疏松后,脂肪含量减少.