二至丸促进围绝经期妇女成骨细胞增殖的分子机制

摘要

Objective To observe the effect of Erzhiwan on the differentiation of human osteoblasts from perimenopausal women,and to analyze the possible molecular mechanism.Methods Cancellous bone of perimenopausal women of different ages who met the inclusion criteria were collected,and divided into four groups:premenopausal normal bone mass group,premenopausal osteopenia group,postmenopausal normal bone mass group and postmenopausal osteoporosis group.Osteoblasts were isolated and cultured in vitro and osteoblast proliferation and differentiation before and after Erzhiwan intervention were evaluated.The ability of osteoblast proliferation was measured by MTT Method.The expression of AKP was measured by color method.The expression of BMP-2 and OCN was measured by ELISA.The expression of the mRNA of BMP-2,OCN and ER-α was measured by SYBR GREEN.Results After Erzhiwan intervention to the osteoblasts of each group,the order of osteoblast proliferation ability,the expression of AKP,BMP-2 and OCN,and the expression of the mRNA of BMP-2,OCN and ER-α from high to low was generally the premenopausal normal bone mass group,the postmenopausal normal bone mass group,the premenopausal osteopenia group and the postmenopausal osteoporosis group.Conclusion To a certain degree,Erzhiwan could multiply osteoblasts and speed up cell differentiation,therefore may play a role in the prevention and treatment of osteoporosis.%目的 观察二至丸对围绝经期妇女成骨细胞分化过程的影响,并探讨其可能的分子机制.方法 随机收集临床不同年龄段符合纳入标准的围绝经期妇女松质骨,分为绝经前骨量正常组、绝经前骨量减少组、绝经后骨量正常组、绝经后骨质疏松组,分离培养体外成骨细胞,对二至丸干预前后成骨细胞的增殖分化,采用MTT法检测各组细胞增殖能力,比色法测AKP,ELISA法检测BMP-2和OCN表达,实时荧光定量PCR-SYBR GREEN法检测核心结合因子BMP-2mRNA、ER-αmRNA、OCNmRNA的表达.结果 二至丸干预后,各组成骨细胞增殖能力、AKP、BMP-2和OCN的表达量及BMP-2mRNA、OCNmRNA和ER-αmRNA的表达量总体由高到低依次为绝经前骨量正常组、绝经后骨量正常组、绝经前骨量减少组、绝经后骨质疏松组,且均较同组干预前显著上升,差异有统计学意义(P＜0.05).结论 二至丸能在一定程度上促进成骨细胞增殖分化,对防治骨质疏松起到积极作用.