Objective To study the effects of ambient monochromatic light on spectral sensi- tivity in rhesus monkeys. Methods Photopie ERG was recorded in six infant rhesus monkeys (2 months old) with different monochromatic light stimuli. Light intensity was attenuated by 0.25 log units from the maximum intensity. The V-Ⅰ (vohage vs intensity) function was set according to the ERG results. The spectral sensitivity curves were plotted by wavelength as the abscissa and log values (1/Ⅰ) as the ordinate ("Ⅰ" defined as the flash intensity while the amplitude of the b-wave reached a maximum of 80 μV). Then the monkeys were raised under three different illumination conditions: red light (peak at 610 nm), blue light (peak at 455 nm) and white light (color temperature 5000 k) (n=2 for each group, 12 h light cycle: 8 am-8 pm). Spectral sensitivity curves were analyzed before and 6 weeks after illumination for each group. Results The monkeys' spectral sensitivity curves peaked at 455～475 nm (short-wavelength), 515～535 nm (middle wavelength) and 595～610 nm (long-wave-length) before illumination. After 6 weeks of illumination, spectral sensitivity curves in the white-light group were almost the same as those before illumination. In the red-light group, a loss of the long- wavelength peak was observed, while no change occurred in the blue-light group except for a broad decline in spectral sensitivity curves. Conclusion Ambient monochromatic light has an effect on the spectral sensitivity of monkeys' eyes and could result in the modification of amplitudes and peak val-ues. These may be associated with the changes in the signal contrast of different chromatic channels.%目的 观察单色光照对恒河猴眼光谱敏感性的影响.方法 2月龄恒河猴6只,明适应下记录闪烁光视网膜电图.刺激光范同为415～650 nm单色光,各单色光峰值间隔约20 nm.光强从最大能量值以0.25 log单位依次递减.记录时,光强由低到高,每一次测试间隔5～10 s,建立各单色光的光强一振幅函数,以振幅为80μV时的光强值记为Ⅰ,以单色光波长值为横坐标,Log(1/Ⅰ)为纵坐标绘制猴眼的光谱敏感曲线.然后将6只恒河猴分别置于红光(610 nm)、蓝光(455 nm)和白光(色温5000 k)照射条件下饲养(光照周期8 am～8 pm),每组2只.分析光照前以及光照6周后的光谱敏感曲线.结果 光照前,恒河猴眼光谱的3个敏感峰值分别在455～475 nm(短波长)、515～535 nm(中波长)和595～610 nm(长波长)3个波段.光照6周后,白光组猴眼光谱曲线与光照前相比,形态和光谱峰值无明显改变;红光组长波长段的敏感峰值降低或消失,而中波长段敏感峰值向长波长段位移,并有增高的趋势;蓝光组的敏感曲线峰值位置基本不变,但整个光谱曲线位置下移,敏感性降低.结论 单色光照能引起幼年恒河猴眼光谱曲线振幅和峰值的改变,其机制可能与色觉通路间信号对比改变有关.
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