胆囊癌组织中血管内皮生长因子C和D的表达及其与淋巴管和血管生成的关系

摘要

Objective To investigate the expression of VEGF-C and VEGF-D and their correlations with lymphangiogenesis and angiogenesis in gallbladder carcinoma.Methods Fifty cases of gallbladder carcinoma with complete clinical and pathological data were analyzed.The expression of VEGF-C and-D,D2-40,CD31 was assayed by immunohistochemical staining.with 10 samples of normal gallbladder tissues away from cancer and 19 samples of chronic cholecystitis as controls.and their correlation with clinieopathological findings were analyzed retrospectively.Results Thirty-two(64.0%)of the 50 gallbladder cancers were positive for VEGF-C protein expression by immunohistochemistry and the positive rate of VEGF-D protein expression was 62.0%(31/50).The protein expression of VEGF-C and VEGF-D in tumor tissues was signifieantly higher than that in normal gallhladder tissues away from the tumor (P<0.05),but no correlation with that in chronic cholecystitis(P<0.05).The VEGF-C expression correlated with the patient age and lymph node metastasis(both P<0.05).The VEGF-D expression only correlated with lymph node metastasis(P<0.05).In the 50 gallbladder eancers,the MLVD was 6.9±3.6 and the MVD was 36.1±12.8.The MLVD in both VEGF-C and-D positive groups wag significantly higher than that in the negative groups(P=0.000),and the lymph node metastasis also increased.MVD in both VEGF-Cand-D positive groups was higher than that in the negative groups(P<0.05),and it was also correlated with tumor differentiation(P<0.05).A significant positive eorrelation was also found between VEGF-C and VEGF-D expression(r=0.498,P<0.01).Conclusion VEGF-C and VEGF-D are involved in the lymphangiogenesis and angiogenesis in gallbladder carcinoma,promote lymph node metastasis of the tumor, and both are important in the regulation of lymphangiogenesis and angiogenesis in this cancer.VEGF-C and VEGF-D are of clinical significance in evaluating lymph node metastatic potency and estimation of prognosis in gallbladder carcinoma.%目的 探讨胆囊癌组织中血管内皮生长因子C(VEGF-C)和VEGF-D的表达及其与淋巴管和血管生成的关系.方法 采用免疫组化法检测50例胆囊癌组织中VEGF-C、VEGF-D、D2-40和CD31的表达,以10例癌旁正常胆囊组织和19例慢性胆囊炎作为对照,并分析其与临床病理诸因素的关系.结果 50例胆囊癌组织中,32例VEGF-C表达阳性,阳性率为64.0%,31例VEGF-D表达阳性,阳性率为62.0%,均高于癌旁正常组织(P<0.05),但与慢性胆囊炎差异无统计学意义(P>0.05).VEGF-C的表达与胆囊癌患者的年龄和淋巴结转移有关,VEGF-D的表达仅与胆囊癌患者的淋巴结转移有关.50例胆囊癌组织的微淋巴管密度(MLVD)和微血管密度(MVD)分别为6.94±3.6和36.1±12.8.VEGF-C阳性组和VEGF-D阳性组的MLVD和MVD均高于阴性组.MLVD与淋巴结转移有关,MVD与淋巴结转移、分化程度有关.VEGF-C与VEGF-D的表达呈正相关(r=0.498,P<0.01).结论 在胆囊癌中,VEGF-C和VEGF-D参与胆囊癌的淋巴生成和血管生成的调节,通过增加瘤周淋巴管的密度促进肿瘤细胞的淋巴结转移.