后Tenon囊下注射曲安奈德对激光光凝后糖尿病大鼠视网膜炎症相关细胞因子表达的影响

摘要

Objective To observe the expression of inflammatory cytokines in diabetic rats received posterior sub-Tenon capsule injection of triamcinolone acetonide (TA) and pan-retinal photocoagulation.Methods A total of 48 Brown Norway rats received intraperitoneal injection of streptozotocin to establish the diabetic model.Diabetic rats were randomly divided into experimental group (20 rats),control group (20 rats) and blank group (8 rats).50 μl TA or saline was injected into the posterior sub-Tenon capsule immediately after the photocoagulation in the experimental group and the control group,respectively.The blank group received no treatment.The mRNA and protein expression level of retinal vascular endothelial growth factor (VEGF),interleukin 6 (IL-6) and tumor necrosis fator-α (TNF-α) were measured by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) at 1,3,7 days after laser photocoagulation.Results The mRNA and protein expression of VEGF,IL-6,TNF-α of the experimental group and control group were significantly higher than the blank group,the difference was statistically significant (P＜0.05).The mRNA and protein expression of VEGF,IL-6 and TNF-α of the experimental group were significantly lower than that of the control group.On day 1 after laser photocoagulation,the mRNA expression of VEGF was not statistically significant in the experimental group and control group (P＞0.05),the mRNA and protein expression of VEGF,IL-6,TNF-α of the two groups were statistically significant in the remaining observing time (P＜0.05).Conclusion Posterior sub-Tenon capsule injection of TA can effectively reduce retinal photocoagulation induced VEGF,IL-6,TNF-α expression.%目的 观察后Tenon囊下注射曲安奈德(TA)对激光光凝后糖尿病大鼠视网膜炎症相关细胞因子表达的影响.方法 雄性Brown Norway健康大鼠48只,通过腹腔注射链脲佐菌素建立糖尿病大鼠模型.将大鼠随机分成实验组、对照组和空白组,分别为20、20、8只大鼠.实验组、对照组大鼠行激光光凝后,给予后Tenon囊下注射TA或生理盐水50μl.空白组不作任何处理.激光光凝后1、3、7d,采用荧光定量聚合酶链反应和酶联免疫吸附试验检测大鼠视网膜中血管内皮生长因子(VEGF)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的mRNA和蛋白表达.结果 激光光凝后1、3、7d,实验组和对照组分别与空白组比较,其VEGF、IL-6、TNF-α mRNA和蛋白表达均明显升高,差异均有统计学意义(P＜0.05).实验组VEGF、IL-6、TNF-α mRNA和蛋白表达均较对照组明显下降.实验组与对照组比较,激光光凝后1d,VEGF mRNA表达间差异无统计学意义(P＞0.05);其余各时间点VEGF、IL-6、TNF-α mRNA和蛋白表达间差异均有统计学意义(P＜0.05).结论 后Tenon囊下注射TA可有效降低激光光凝导致的糖尿病大鼠VEGF、IL-6、TNF-α表达水平的升高.