目的:从羊小肠提取抗菌肽并对其抑菌作用进行研究,寻找廉价易得的广谱高效天然抗菌肽.方法:葡聚糖凝胶分离及反相高效液相色谱法提纯羊小肠活性抗菌肽;采用琼脂平板打孔法进行抑菌效果的测定;SDS-PAGE电泳分析相对分子质量.结果:反相高效液相色谱法分离出现3个主峰,其中之一对大肠杆菌和金葡球菌有较高抑菌活性(蛋白浓度分别为38,19,10 mg·mL-1);相对分子质量约为40 Ku.结论:实验得到的抗菌肽材料易得,对G-菌和G+菌均有较强的抑制作用.%Objective: To isolate antibacterial peptides from small intestines of sheep, and investigate their antibacterial activities. Methods: The gel filtration chromatography with Sephadex G-25 was used to separate different components from the small intestine extracts of sheep. The separated components were analyzed by agar diffusion plate method to determine their antibacterial activities. The active component was further separated by reversed-phase high-performance liquid chromatography. The purity and molecular weight of the peptide with antibacterial activity was determined by SDS-PAGE. Results: The antibacterial peptides separated from sheep small intestine showed a highly activity to inhibit growth of Escherichia coli and Staphylococcus aureus at protein concentrations of 38, 19, 10 mg·mL-1. Its molecular weight was about 40 Ku. Conclusion: The new antibacterial peptides are easily obtained, and has significant antimicrobial activity.
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