高效液相色谱法测定人血浆氟比洛芬对映体浓度

摘要

目的:建立用高效液相色谱法测定血浆氟比洛芬对映体浓度的方法.方法:本试验采用环己烷提取血浆样品中氟比洛芬,色谱柱为Chirobiotic V(250 mm×4.6 mm,5μm),流动相为甲醇-冰醋酸-三乙胺(100∶0.01∶0.01),检测波长为250 nm,内标为S-酮洛芬.结果:各组分之间没有干扰,分离度良好.血浆中R-氟比洛芬和S-氟比洛芬浓度在30 ～1 000 ng·mL-1范围内线性关系良好.两个对映体相对回收率分别为98.6％ ～ 100.2％和98.7％ ～ 101.2％,绝对回收率分别为76.1％～81.6％和76.6％ ～ 80.5％,日内RSD分别为3.9％ ～5.7％和4.1％～6.2％,日间RSD分别为5.8％ ～7.5％和6.1％ ～7.4％.结论:该方法操作快速简便,重现性好、灵敏度高,可满足血浆氟比洛芬对映体浓度测定及药代动力学研究的需要.%Objective :To establish a method for the determination of plasma levels of enantiomers of flurbi-profen. Methods; Flurbiprofen was extracted with cyclohexane. HPLC was performed on a Chirobiotic column with methanol-aceticacid-triethylamine( 100: 0. 01 : 0. 01 ) as mobile phase. Detection wavelength was 250 nm. S-Ketopro-fen was used as the internal standard. Results:Under this chromatographic condition,R-flurbiprofen , S-flurbiprofen and S-Ketoprofen had no interference with each other. The calibration curve was linear in the range of 30 ～ 1 000 ng-mL for R-flurbiprofen and S-flurbiprofen. The relative recoveries of R-flurbiprofen and S-flurbiprofen were 98. 6％ ～ 100. 2％ and 98. 7％ ～ 101. 2％ , respectively. The absolute recoveries of R-flurbiprofen and S-fiurbipro-fen were 76. 1％ ～ 81.6％ and 76.6％ ～ 80.5％ , respectively. The intraday RSDs were 3.9％ ～ 5.7％ and 4. 1％ ～ 6.2％ , and interday RSDs were 5. 8％ ～ 7. 5％ and 6. 1％ ～ 7.4％ , respectively. Conclusion:This method is rapid and simple,with high sensitivity and good repeatability,it can be used for the determination of flurbiprofen enantiomers in human plasma and their pharmacokinetic study.