目的:建立一种超高效液相色谱法同时测定丹蛭降糖胶囊中丹皮酚、芍药苷的含量.方法:色谱柱为Waters Acquity UPLC BEH C18(50mm×2.1mm,1.7μm)柱,以乙腈-0.1％磷酸为流动相进行梯度洗脱,流速为0.25Ml·min-1,柱温为30℃,检测波长为230nm.结果:丹皮酚和芍药苷的线性范围分别为0.653 ～1.959μg(r=0.9997)和0.108～0.324μg(r=0.9999),平均加样回收率分别为99.46％,RSD=0.46％(n=6)和99.19％,RSD=0.49％(n=6).结论:新建方法简便、快速、准确、易行,可用于控制制剂质量.%Objective: To investigate whether boningmycin, a new member of bleomyc in family, overcomes multidrug resistance of tumor cells. Methods; The doxorubicin-resistant human breast cancer MCF-7 cells( MCF-7/DOX ) and vincristine-resistant human oral epithelial cells( KBV200 ) and related sensitive cells were chosen for the experiments. MTT reduction assay was used to detect the inhibitory effect on cell proliferation. Cell cycle distribution and accumulation of intracellular reactive oxygen species (ROS) were analyzed with flow cytometry. Chromatin condensation was detected by a fluorescent microscope after Hoechst 33342 staining. Protein expression was detected by Western blot. Results: MTT results showed that IC50 values of MCF-7/DOX cells and MCF-7 cells inhibited by boningmycin were 0.17 and 0. 10 μmol·L~-1, respectively, and those of KBV200 cells and KB cells were 0. 38 and 0. 21 μmol·L-1, respectively, showing no cross resistance to boningmycin in resistant cells. Furthermore, the arrest at G2/M phase and increase in ROS level were detected by flow cytometry. Boningmycin induced the chroma-tin condensation, apoptosis and decreased expression of multidrug resistance-associated P-glycoprotein detected by Western blotting. Conclusion; Boningmycin can overcome the multidrug resistance in tumor cells and its mechanisms are related with induction of apoptosis and reduction of P-glycoprotein expression.
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