白念珠菌破壁方法的研究应用进展

摘要

Cell wall as a special and necessary cell structure of fungus itˊs hard to make it fully broken in a simple way There-fore,to achieve the ideal wall-breaking effect is one of the key steps in the study of Candida albicans In extraction such as RNA, DNA and protein components of Candida albicans ,in order to obtain sufficient and stable experimental samples,the researchers shoulduse different methods breaking the cell wall according to different research purposes This paper summarizes a variety of Candida albicans wall-breaking methods,so as to provide applicable and efficient solution of wall-breaking for related research in Candida albicans.%细胞壁作为真菌中特殊和必须的细胞结构，相对于哺乳动物的细胞膜更加坚硬，难以用简单的方法使其充分破碎。因此，达到理想的破壁效果是白念珠菌研究中的关键步骤之一。在提取白念珠菌RNA、DNA和蛋白质等细胞组分的过程中，为获得足量和稳定的实验样品。该文对多种白念珠菌破壁方法作一综述，以便为白念珠菌相关研究提供适用、高效的破壁方案。