异源蓝舌病毒dsRNA与PolyI:C诱导人源体细胞产生IFN-β的比较研究

摘要

目的 探讨异源动物蓝舌病毒dsRNA诱导人源细胞产生干扰素(IFN)的能力.方法 借助体外培养系统,利用ELISA技术检测IFN诱导剂多聚次黄苷酸-胞苷酸(PolyI:C)、蓝舌病毒(BTV)及BTV dsRNA 3种不同处理因素作用后,培养细胞上清液中IFN-β的含量,以比较研究其诱导人肺癌细胞A549和人胚肺细胞HEL产生IFN-β的能力和特征.结果 这3种因子均能诱导人肺癌A549细胞和人正常肺细胞HEL产生IFN-β,BTV dsRNA诱导IFN-β产生的能力显著高于Poly I:C和BTV.浓度高的BTV dsRNA诱导细胞产生的IFN-β水平高,说明二者之间存在剂量依赖性.BTVdsRNA对不同性质的人源细胞(人肺癌A549细胞、人正常肺细胞HEL)具有不同的诱导产生IFN-β的能力,诱导人正常肺细胞HEL产生IFN-β的能力显著地高于诱导人肺癌细胞A549产生IFN-β的能力(P<0.05).结论 裸露的BTV dsRNA分子能有效地诱导人源细胞产生IFN-β;结合BTV和dsRNA对人的安全性以及人类正在努力寻找新的内源性IFN诱导剂的事实,可以推断BTV dsRNA具有发展成内源性IFN诱导剂之潜能.%Objective To investigate the capability of bluetongue virus(BTV)dsRNA inducing IFN-β from human cells.Methods Artificial complex interfemn inducer polyinosinic-polycytidylic acid (PolyI:C).BTV and BTV dsRNA were added to A549(human lung cancer cell)and HEL(human lung normal cells)culture system in difierent concentrations.IFN-β in culture median was detected by ELISA.Results Though all of the 3 reagents could induce IFN-β,BTV dsRNA significanay induced the highest level of IFN-β.The production of IFN-β was induced by BTV dsRNA in dose dependence.BTV dsRNA induced IFN-β level from HEL Was higher than that from A549(P<0.05).Conclusion BTV dsRNA Can induce IFN-β from human cells effectively,which shows its potential of an endogenous IFN-β inducer.