正常中国人及肥胖患者解偶联蛋白1基因-3826 A/G多态性的研究

摘要

Objective To investigate the-3826A/G polymorphism in the promoter of the uncoupling protein-1(UCP1) gene and its relations to obesity in Chinese population. Methods Three hundred and eighty-four subjects (257 non-obese and 127 obese individuals) from a population of Chinese Han nationality in Chengdu area were studied using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs). Serum lipids were measured by enzymatic kits and apolipoproteins AⅠ, AⅡ, B100, CⅡ,CⅢ and E were measured by the RID kits. Results The frequencies of A and G alleles at-3826A/G site in obese and non-obese groups were 0.508 and 0. 492, and 0. 467 and 0. 533, respectively. It showed no significant difference in allele frequencies between non-obese and obese groups (P>0.05). In the obese group, subjects with genotype GG had higher serum apo B100 concentrations, and those with genotype AG had higher apo CⅡ and apo CⅢ levels, than those with genotype AA, respectively (P<0.05). In non-obese male subgroup, subjects with genotype GG had lower serum HDL-C and apo AⅠ levels than those with genotype AA, respectively (P<0.05), whereas those with genotype AG had lower apo AⅡ levels than those with genotype AA. In addition, in obese males with genotype GG had elevated apo B100 levels compared with those with genotype AA, whereas in obese females with genotype GG had decreased apo AⅠ levels and genotype AG had increased apo CⅡ and apo CⅢ levels compared with those with genotype AG and AA, respectively (P<0.05). Conclusion -3826A/G polymorphism in the promoter of the uncoupling protein-1 gene was not associated with obesity in Chinese Han population of Chengdu area. It may be associated with serum HDL-C,apo AⅠ and apo B100 levels in non-obese and/or obese subjects of certain genders.%目的 研究解偶联蛋白1(uncoupling protein 1,UCP1)基因-3826 A/G多态性是否与中国人肥胖有关联,为探讨肥胖的分子遗传基础提供依据.方法 应用聚合酶链反应-限制性片段长度多态性分析法,对成都地区384名汉族人(257例非肥胖者及127例肥胖者)UCP1基因-3826 A/G多态性位点进行分析,采用酶法和单向免疫扩散法对血脂和载脂蛋白水平进行测定.结果 UCP1基因-3826 A/G多态位点A、G等位基因频率在肥胖组为0.508、0.492,在非肥胖组为0.467、0.533,两组间等位基因的频率差异无统计学意义(P>0.05).-3826 A/G位点在肥胖组GG基因型携带者血清载脂蛋白(apolipoprotein,apo)B100水平高于AA基因型者(P<0.05),AG型者apo CⅡ、apo CⅢ水平均分别高于AA基因型携带者(P<0.05).在非肥胖男性亚组,GG纯合基因型携带者血清高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)及apoAⅠ水平显著低于AA基因型携带者(P<0.05);AG型者apoAⅡ水平低于AA基因型者.在肥胖男性亚组,GG基因型携带者血清apo B100水平较AA型者显著升高(P<0.05);在肥胖女性亚组,GG基因型者apo AⅠ水平低于AG型者,AG型者apoCⅡ、apoCⅢ水平均分别高于AA型者(P<0.05).结论 UCP1基因-3826A/G多态性与成都地区中国汉族人肥胖无关联,但与血清HDL-C及apoAⅠ、apoB100等载脂蛋白水平含量有关,且具有性别差异.