固醇调节元件结合蛋白1c基因多态性rs2297508、rs11868035与甘肃汉族、东乡族人群2型糖尿病的相关性

摘要

目的 探讨固醇调节元件结合蛋白1c(sterol regulatory element binding protein-lc,SREBP-lc)基因多态性rs2297508、rs11868035在甘肃汉族、东乡族人群中的分布及其与2型糖尿病(type 2diabetes mellitus,T2DM)的相关性.方法 选择汉族2型糖尿病患者342例以及正常对照343人,东乡族2型糖尿病患者218例以及正常对照238人,采用聚合酶链反应-变性高效液相色谱法检测SREBP-1c基因型,采用氧化酶法或放免法测定血糖、胰岛素及血脂水平.采用卡方检验进行统计学分析.结果 SREBP-lc基因多态位点rs2297508、rs11868035在汉族和东乡族正常对照者中的基因型和等位基因频率分布差异无统计学意义(P＞0.05).上述位点在汉族、东乡族人群2型糖尿病患者C等位基因和CC基因型频率均明显高于对照组,差异均有统计学意义(P＜0.01).在汉族对照组中,rs2297508的C等位基因携带者的低密度脂蛋白胆固醇水平明显高于GG者,其差异有统计学意义(P＜o.05).在东乡族对照组中,CC基因型的低密度脂蛋白胆固醇水平明显高于GG者,其差异有统计学意义(P＜0.05).结论 SREBP-lc基因多态性rs2297508、rs11868035在甘肃汉族和东乡族人群中均与2型糖尿病发病存在关联.C等位基因可能是罹患2型糖尿病的危险因素之一.上述多态性在汉族和东乡族人群中的分布并无差异.SREBP-le基因多态性rs2297508可能与低密度脂蛋白胆固醇升高有关.%[Objective]To assess the association between single nucleoride polymorphisms (SNP)rs2297508 and rs11868035 of sterol regulatory element binding protein-lc (SREBP-lc)gene and type 2 diabetes mellitus (T2DM) in Han and Dongxiang populations in Gansu.[Methods] A total of 342 patients with T2DM and 343 healthy controls of Han ethnics and 218 patients with type 2 diabetes and 238 healthy controls of Dongxiang ethnics were enrolled during 2006 and 2009.Genotypes of rs2297508 and rs11868035 were determined by polymerase chain reaction-denaturing high-performance liquid chromatography (PCR-DHPLC).Plasma levels of lipid,glucose and insulin in all subjects were assayed by biochemical techniques.x2 test was used for data analysis.[Results] The genotypic and allelic frequencies of rs2297508 and rs11868035 polymorphisms did not differ significantly in the control groups of Han and Dongxiang ethnics (P＞0.05).But those of T2DM patients were significantly higher than in the controls (P＜0.01).Plasma low density lipoprotein cholesterol(LDL C) levels for carriers of C allele for rs2297508 were significantly higher than those of non-carriers from the control group of Han ethnics (P＜0.05).That of carriers of CC genotypes for rs11868035 was significantly higher than carriers of GG genotype in the control group of Dongxiang ethnics (P＜0.05).[Conclusion] SNP rs2297508 and rs11868035 of SREBP-lc gene may be associated with T2DM,and C allele appears to be a risk factor for T2DM in Gansu Han or Dongxiang ethnics.Han and Dongxiang ethnics did not differ significantly in terms of genotypic and allelic frequencies for the two SNPs.SNP rs2297508 of SREBP lc gene may be associated with increased plasma levels of LDL-C in both Han or Dongxiang ethnics.