IL-18在慢性HBV感染后不同转归患者肝脏组织中的表达及意义

摘要

Objective To investigate the relationship and clinical significance between interleukin 18 (IL-18) expression in liver tissues and CHB patients with different prognosis. Methods Total of 230 CHB patients in the Second Affiliated Hospital of Anhui Medical University were enrolled. The tissue specimens were obtained by liver biopsy from all patients, and 21 normal liver specimens were also collected as controls. The liver function and liver inflammation grades and fibrosis stages were assessed. Immunohistochemical method was used to detect the expression of IL-18 in liver tissues. Spearman rank correlation was used to analyze the correlations between IL-18 expression and the above indicators. Results IL-18 was mainly expressed in the cytoplasm of hepatocytes. The difference of IL-18 expression level between CHB patients and healthy people has statistically significance (P < 0.05). In patients with HBV infection, the expressions of IL-18 are gradually increased with elevated levels of alanine aminotransferase, aggravating the degrees of inflammation and fibrosis (r = 0.184, 0.169, 0.085, all P < 0.05). Conclusions The expression of IL-18 may be involved in liver injury and disease progression in CHB.%目的：探讨白细胞介素-18（IL-18）的表达与慢性HBV感染后不同疾病转归患者的相关性及其临床意义。方法收集230例慢性HBV感染后不同疾病患者肝脏组织和临床资料，采用半定量SP免疫组织化学法检测HBV感染者肝组织中IL-18的表达情况，并与患者的临床生物化学指标、肝脏炎症分级和纤维化分期进行比较，分析肝脏组织中IL-18表达与上述指标的相关性。结果 IL-18在肝脏组织中以肝细胞细胞质表达为主，半定量分析结果显示，在健康者、CHB、肝硬化、HBV相关性肝癌患者肝组织中的IL-18表达，差异均有统计学意义（P ＜0.05）；IL-18在HBV感染者肝组织中的表达与ALT水平、肝脏炎症及纤维化程度均呈正相关（r =0.184、0.169、0.085，P =0.015、0.021、0.032）。结论IL-18表达强弱与HBV感染的免疫发病机制有关，IL-18参与了HBV感染的慢性化和疾病的转归。