胃癌组织中MVD计数及CD44v6,VEGF表达的意义

摘要

Objective To investigate microvessel density (MVD) and the expression of CD44v6 and VEGF in primary gastric carcinoma and to evaluate its relationship with the tumor oncogenesis and biological malignant behavior.Methods The specimens from 59 cases of primary gastric carcinoma, 10 cases of dysplastic gastric mucosa and 13 cases of chronic superficial gastritis were analyzed by HE staining.MaxVisionTM immunohistochemical method was used.Results The positive results of(66.1%)and VEGF(71.2%)in primary gastric carcinoma were significantly higher in gastric carcinoma compared with that in control group(P＜0.05),MVD was significantly higher in gastric carcinoma compared with that in control group(P＜0.05),There were also correlation between CD44v6,VEGF,MVD and serosal invasion,metastasis of gastric carcinoma(P＜0.05),There was close positive correlation between the expression of CD44v6,VEGF and MVD respectively.Conclusion MVD,CD44v6 and VEGF are valuable indicators for the biological characteristics of primary gastric carcinoma and helpful in distinguishing malignant from benign tumors.%目的 研究CD44v6,VEGF基因蛋白和胃癌微血管密度与胃癌的发生、发展及生物学行为的关系,并探讨三者的相关性.方法 应用免疫组化MaxVisionTM染色方法检测59例胃癌,10例胃粘膜不典型增生病变和13例慢性胃炎组织的CD44v6,VEGF,CD34的表达.结果 胃癌组织中CD44v6和VEGF的阳性表达率分别为66.1%和71.2%,均高于不典型增生组及慢性胃炎组(P＜0.05).胃癌中MVD值高于不典型增生组及慢性胃炎组(P＜0.05),CD44v6和VEGF的表达及MVD与是否浸润浆膜、淋巴结转移及TNM分期有关(P<0.05).胃癌MVD计数分别与CD44v6表达,VEGF的表达存在正相关.结论 检测CD44v6和VEGF的表达和MVD计数是反映胃癌生物学特性良好的指标,对判断胃癌的浸润转移具有一定的临床意义.