尼莫地平对氧化低密度脂蛋白诱导人血管内皮细胞凋亡的影响

摘要

Objective To study the effect to N in odipine on vascular endotheEal cell apoptosis induced by varfaus concentrations of ox—LD L .M eihods Cultured vascular endotheEal (ECV304) were treated with varfaus concentrations o of oxriDL and N inodpine for 624houis .A tranan ission electron m icroscope w as em pbyed to obseive 1he cell apoptisis .The apoptosis rate of EC V 304 cell w as anayied with flow cytom eter.The concentration of Ca was detected by a laser scanning confocalm icroscope (LCSM ) .Results ox—LDL was able to induce apoptisis in human EC V 304 cells. N in odipine could inhibit apoptosis of EC V 304 cells. Conclusion Ni-m odipine could protect EC V 304 cells from various concentrations of ox—LDL induced apoptosis.%目的 研究尼莫地平对不同浓度氧化低密度脂蛋白诱导的人血管内皮细胞凋亡的影响.方法 在人脐静脉内皮细胞株ECV304培养基中加入不同浓度氧化低密度脂蛋白和尼莫地平(1.2 μg/ml),作用6-24 h.透射电镜观察内皮细胞ECV304的凋亡情况;流式细胞术(FCM)检测细胞凋亡率;用激光共聚焦显微镜检测钙离子浓度.结果 不同浓度氧化低密度脂蛋白能明显诱导培养的内皮细胞发生凋亡,尼莫地平可显著降低氧化低密度脂蛋白诱导的内皮细胞凋亡率(P<0.01).结论 尼莫地平对氧化低密度脂蛋白诱导的人血管内皮细胞凋亡具有保护作用.