目的 探讨组织型转谷氨酰胺酶(tTG)在单侧输尿管梗阻(UUO)大鼠肾间质中的表达及其意义.方法 雄性Wistar大鼠随机分为假手术组和模型组,采用左侧输尿管结扎术制备肾间质纤维化模型.术后第2周末处死各组大鼠,采用HE和Masson染色观察肾组织病变;采用免疫组化法检测肾间质中tTG和纤维连接蛋白(FN)的表达;采用RT-PCR技术检测tTG mRNA表达.结果 HE显示,模型组大鼠肾间质增宽,炎细胞浸润,纤维组织增多,肾小管明显扩张.Masson染色可见模型组大鼠肾间质胶原明显增多.模型组tTG和FN的基因和蛋白表达均明显高于假手术组(P＜0.01),并且tTG的蛋白水平与FN水平呈显著正相关(r=0.734,P ＜0.05).结论 tTG可能通过交联细胞外基质蛋白,抵抗降解,参与UUO大鼠肾间质纤维化的发生发展.%Objective To investigate the expression of tissue transglutaminase (tTG) in unilateral ureteral obstruction (UUO) rats .Methods The animal models of unilateral ureteral obstruction (UUO ) were used .Wistar rats were randomly divided into the Sham-operated group and the UUO group .The rats were sacrificed at day 14 .Histologicalchanges in renal tubular interstitium were ob-served with HE and Masson staining ,and the mRNA and protein levels of tTG and FN were detected by RT-PCR and immunohistoche-mistry .Results Massive accumulation of collagen in tubulointerstitium leads to renal fibrosis .Compared with the sham group ,the ex-pression of tTG and FN were significantly increased ( ρ＜0 .01) ,and the levels of tTG protein had positive correlation with the levels of FN protein ( γ=0 .734, ρ＜0 .05) .Conclusion tTG may play a important role in the pathogensis of renal tubulointerstitial fibrosis .
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