目的 探讨去除高丰度蛋白质对脑脊液差异蛋白鉴定的影响.方法 应用Cibacron-blue-3G-A亲和层析柱去除脑脊液中含量最多的高丰度蛋白质-白蛋白,然后对去除白蛋白前后的脑脊液样本,以及经过Cibacron-blue-3G-A亲和层析柱吸附后洗脱下来的脑脊液样本,分别进行双向凝胶电泳,对比分析各样本差异蛋白点的分布情况.结果 Cibacron-blue-3G-A亲和层析柱通过结合去除了脑脊液中的大部分白蛋白,但是同时也去除了一些其它可能有意义的蛋白质,而且在去除白蛋白后,一些在白蛋白存在时丰度显现并不是很高的蛋白质丰度相对增加,同样干扰了周边低丰度蛋白的检出.结论 亲和层析柱无法特异性去除脑脊液中的高丰度蛋白质,丢失的蛋白信息可能影响后续疾病相关差异蛋白的鉴定.%Objective To explore the effects of high-abundance proteins depletion on ccrcbrospinai fluid (CSF) differentiated protein Identification. Methods The most high-abundance protein-albumin was removed from CSF by Ciba-cron-b1uc-3G-A affinity chromatography. Then,CSF samples with and without albumin,also the bound proteins on Ci-bacron-bluc-3G-A affinity chromatography were respectively compared by two-dimensional gel clcctrophorcsis. Results Cibacron-bluc-3G-A affinity chromatography could combine and remove most of CSF albumin. However,lots of probably meaningful proteins were lost after albumin depletion. Also some other protein abundance increased, which interfered in low-abundance CSF proteins display around them. Conclusion Affinity chromatography could not specially remove high-abundance CSF proteins. Lots of proteins were lost when albumin depletion, which will affect disease-related proteins identification.
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