目的：探讨小柴胡汤对 C6胶质瘤大鼠 ESM-1和 VEGF 蛋白表达水平的影响。方法清洁级 Wistar 老年雄性大鼠18月龄，n＝60只，右前肢腋窝皮下接种2×106/20μl 的细胞悬液；然后随机分为6组：肿瘤对照组、阳性药A 组（替莫唑胺；50 mg/Kg）、阳性药 B 组（替莫唑胺；25 mg/Kg）、小柴胡汤 C 组（50 g/Kg）、小柴胡汤 D 组（20 g/Kg）、小柴胡汤 E 组（10 g/Kg）；每组均分别灌胃给予生理盐水、阳性药 A、阳性药 B、小柴胡汤 C、小柴胡汤 D 和小柴胡汤E，连续服药5 d，休息4 d，共给药3次，于最后一次给药后2 d 处死动物，眼球取血及取肿瘤组织。采用 ELISA 酶联免疫法检测大鼠血清及肿瘤组织中 ESM-1和 VEGF 的蛋白含量；采用免疫组织化学方法检测大鼠肿瘤组织中 ESM-1和 VEGF 的蛋白表达水平。结果与肿瘤对照组比较，小柴胡汤各剂量组大鼠血清及肿瘤组织中 ESM-1、VEGF 蛋白含量和蛋白表达水平均降低，蛋白含量随剂量增长降低趋势明显差异有统计学意义（P ＜0．01），蛋白表达水平 C组、D 组降低较为显著但二者之间差异无统计学意义（P ＞0．05）；阳性药各剂量组大鼠血清及肿瘤组织中 ESM-1、VEGF 蛋白含量和蛋白水平亦均降低，且随剂量增长降低趋势明显差异亦有统计学意义（P ＜0．01）；阳性药 A 组、小柴胡汤 C 组和 D 组这三组之间在肿瘤组织蛋白表达水平上差异无统计学意义（P ＞0．05）。结论与阳性药各剂量组的治疗机制相一致，小柴胡汤各剂量组均能通过下调 ESM-1和 VEGF 的水平治疗 C6胶质瘤，高剂量组作用最为显著，其作用机制有待于进一步探讨。%Objective To investigate Xiaochaihutang impact on ESM-1 and VEGF protein expression in C6 glioma in rats.Methods Wistar male rats aged 18 months,n=60,with the right forelimb axillary subcutaneous inoculation of cell suspension 2×106/20 μl;then randomly divided into six groups :control group tumors ,positive drug group A(temo-zolomide;50 mg/Kg),positive drug group B(temozolomide;50 mg/Kg),Xiaochaihutang group C(50 mg/Kg),Xi-aochaihutang group d(20 mg/Kg),Xiaochaihutang group E(10 mg/Kg);each group were orally administered saline, positive drug A,positive drug B,Xiaochaihutang C,Xiaochaihutang D and E,continuous medication 5 d,rest 4 d,were to drug three times in the last 2 d animals were sacrificed after administration ,blood and tumor tissue.Using ELISA to detect the protein content of ESM-1 and VEGF in serum and tumor tissue,Immunohistochemistry was used to detect the protein expression of ESM-1 and VEGF in rat tumor tissue.Results Compared with the control group tumors,the protein content and protein expression of ESM-1 and VEGF in Xiaochaihutang each dose group on serum and tumor tis-sue were decreased ,protein content reduced the growth trend with dose significant difference was statistically(P <0.01);protein expression levels group C,D group decreased more significant but the difference between them was not statistically sifnificant(P >0.05);positive serum and tumor tissue of rats in each dose group in protein content and protein levels of ESM-1 and VEGF were lower,and with lower doses of growth trend evident difference was also statis-tically significant(P <0.01);positive drug group A,Xiaochaihutang group C and D between the three groups ,the difference was not statistically significant(P >0.05).Conclusion Consistent with mechanism of positive drug treat-ment each dose group ,Xiaochaihutang each dose group could cut through the level of ESM-1 and VEGF treatment C6 glioma high-dose group the most significant effect,the mechanism needs further explored.
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