七叶皂苷钠对脑缺血再灌注损伤大鼠炎性细胞因子的影响

摘要

Objective To investigate the effect of sodium aescinate (SA)on inflammatory cytokines in rats with cerebral ischemia/reperfusion (CI/R)injury.Methods Rat model of CI/R was prepared by four vessel occlusion for 10 min fol owed by 12 hour reperfusion.Rats were then randomly divided into 5 groups (n=10),namely sham group,model group,SA high,medium and low dose groups (10,20 and 40 mg/kg).Drugs were i.p.administered once a day for 3 days before the operation and once 1 hour after the operation,respectively.The levels of tumor necrosis factor α(TNF α),interleukin (IL) 1βin brain tissue homogenate were measured by ELISA after 12 hour reperfusion.Resluts The neurological behavior scores in SA high dose group and SA medium dose group were lower than that in model group(P<0.05).The levels of brain TNF αin SA groups were significantly lower than that in model group (P<0.05).The levels of brain IL 1βin SA high dose group and SA medium dose group were lower than that in model group(P<0.05).Conclusion SA pretreatment can inhibit inflammatory cytokines in brain tissue in rats with CI/R injury.%目的：观察七叶皂苷钠对脑缺血再灌注(CI/R)损伤大鼠脑组织中炎性细胞因子的影响,探讨其作用机制。方法采用四血管阻塞10 min的方法建立 CI/R大鼠模型,并进行12 h再灌注,试验分为假手术组、模型组及七叶皂苷钠高、中、低剂量组(10 mg/kg、20 mg/kg、40 mg/kg),术前3 d开始腹腔注射给药,每天1次,术后1 h给药1次。再灌注12 h后,ELISA法检测脑匀浆中肿瘤坏死因子α(TNF α)和白介素1β(IL 1β)水平。结果七叶皂苷钠高、中剂量可显著降低CI/R损伤大鼠的神经行为学评分,与模型组比较差异有统计学意义(P<0.05)。七叶皂苷钠各剂量均能降低 CI/R损伤大鼠脑组织的 TNF α水平,高、中剂量可明显降低脑组织IL 1β的表达水平,与模型组比较差异均有统计学意义(P<0.05)。结论七叶皂苷钠预处理对CI/R损伤大鼠的脑保护作用可能与其减少炎性细胞因子的过度释放有关。