丹参注射液对脑梗死大鼠双侧脑室室管膜下区神经干细胞分化作用的影响

摘要

目的：观察丹参注射液对脑梗死大鼠双侧脑室室管膜下区(SVZ)内神经干细胞(NSCs)分化作用的影响。方法成功制作大脑中动脉闭塞(MCAO)模型，随机分为丹参注射液组、生理盐水组，每组12只，每组再分为治疗7 d、14 d两个亚组。治疗前和治疗后7 d、14 d采用改良神经功能损害评分(mNSS)评定各组大鼠神经功能，另随机选取12只 SD大鼠为假手术组。以免疫荧光染色法检测 SVZ内溴脱氧尿嘧啶核苷(BrdU)/神经特异核蛋白(NeuN)、胶质纤维酸性蛋白(GFAP)/ NeuN双标阳性细胞。结果治疗7 d和14 d后，丹参注射液组 mNSS评分较生理盐水组下降，差异有统计学意义(P<0.05)；各时间点丹参注射液组双侧 SVZ内 BrdU/NeuN双标阳性细胞较其他两组增高，差异有统计学意义(P<0.05)。结论丹参注射液能够促进 NSCs向神经元分化及改善神经功能，对脑梗死后神经重塑发挥了积极作用。%Objective To investigate the effects of Danshen injection on differentiation of neural stem cel s (NSCs)in bilateral ventri-cle subventricular zone(SVZ)in rats with cerebral infarction.Methods The 24 successful middle cerebral artery occlusion(MCAO) adult male Sprague Dawley (SD)rat models were randomly devided into a Danshen injection group,a saline group. In each group, 12 MCAO rats were randomly al ocated into groups(6 rats in each subgroups).Neurological deficits were evaluated using the modified neurological severity score(mNSS)before treatment and on the 7 th,14 th day after treatment.Another randomly selected 12 normal rats served as sham operation group. BrdU/NeuN,NeuN/GFAP double positive cel s were detected by immunofluorescence tech-niques.Results After 7 d,14 d treatment,Danshen injection group rats’mNSS was decreased compared with saline group,the difference was statistical y significant(P<0.05);each time,the number of BrdU+ /NeuN+ cel s in Danshen injection group were in-creased than the other two groups,the difference was statistical y significant(P<0.05).Conclusion Danshen injection can promote differentiation of NSCs into neurons and improve neurological function after cerebral infarction,play a positive role in neural remodeling.