过度运动对大鼠肾损伤敏感标志物的影响及人参总皂苷的干预研究

摘要

Objective : We investigate the changes of some sensitive hiomarkers in rats undertaking excessive - exercise, and the effects of Ginsenoside ( GS ) which was a product from a plant herb - Ginsen.Methods : Male Sprague - Dawley rats divided into five groups: control group( CTL ), over - exercise group( two sub - group : M2 and M24 ); Ginsenoside group ( two sub - group: G2 and C24 ).The animal model of excessive - exercise induced AKI was established by exhaustively running on the treadmill according protocol.We administrated with GS ( 100g/kg· d ) intragastric ahead the G2 and G24 rats took exhausting running.We collected urine 2h or 24h after excessive - exercise , and measured their albumin, neutrophil gelatinase - associated lipocalin ( NGAL ) and N - acetyl - d - glucosaminidase( NAG ).We collected blood to test the renal function ( BUN, serum creatinine ), superoxide dismutase ( SOD ) and malondialdehyde ( MDA ).The hlood and kidney Angiotensin Ⅱ ( Ang Ⅱ ) were measured by radioimmunoassay ( RIA ).We observed the renal pathology by Microscope after HE staining.Results : Over - exercise induced the urine albumin and NAG excretion increasing, the BUN and blood NGAL increased too.Compared with the blood and kidney Ang Ⅱ increased, the serum and kidney tissue SOD decreased, but MDA increased at the same time.We ohserved a part of the renal tuhular epithelial cells swelling and ablating.After treatment by GS, the excretion of urine albumin and NAC reduced.BUN and NGAL in hlood decreased.GS decreased the kidney Angiotensin Ⅱ and MDA level, but increased the SOD level in blood or kidney tissue, thus, ameliorate the tubular injury.Conclusion : Ginsenoside protect the tuhular injury induced by the over - exercise in rats, which mechanism might reduce the Ang Ⅱ and anti - oxidative stress in the kidney.%目的:探讨过度运动对大鼠肾组织及肾损伤敏感标志物的影响,及人参总皂苷的防治作用.方法:SD大鼠随机分为对照组(CTL)、过度运动组(M2、M24两个亚组),人参总皂苷组(G2、G24两个亚组),每组7只.采用大鼠运动跑台建立过度运动致大鼠急性肾损伤模型,人参总皂苷组于运动前1周给予人参总皂苷100 mg·kg-1·d-1灌胃,模型组和对照组给予等体积蒸馏水灌胃,分别于运动后2 h,24 h检测尿蛋白、中性粒细胞明胶酶载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)、N-乙酰β-D氨基葡萄糖苷酶(NAG酶),腹主动脉采血检测肾功能变化,检测血浆及肾组织中血管紧张素Ⅱ(AngⅡ)浓度、SOD活性和MDA含量;光镜下观察肾组织病理改变.结果:过度运动可引起大鼠尿蛋白、尿NAG酶和血尿素氮、血NGAL水平升高,血浆及肾组织中AngⅡ浓度升高;伴随血清及肾组织中SOD活性降低、MDA含量升高,病理学光镜可见少量肾小管上皮细胞肿胀和脱落.人参总皂苷可明显降低大鼠过度运动后尿素氮水平,减少过度运动后蛋白尿和尿NAG酶排泄,抑制运动后肾组织AngⅡ升高,升高血清及肾组织中SOD活性,降低MDA含量(P<0.05),改善运动后肾脏病理损伤.结论:过度运动可引起尿蛋白及尿酶升高,及肾小管损伤;人参总皂苷对大鼠过度运动后AKI具有预防保护作用,其机制可能与抗氧化应激及减少运动后肾组织AngⅡ含量有关.