复方麦芽丸对多囊卵巢综合征大鼠雄激素受体及胰岛素受体基因表达的影响

摘要

目的：观察复方麦芽丸对多囊卵巢综合征（PCOS）大鼠雄激素受体（AR）、胰岛素受体（INSR）基因表达的影响，探讨其相关作用机制。方法采用来曲唑诱导建立PCOS大鼠模型。将大鼠随机分为正常组、模型组、达英-35组及复方麦芽丸低、中、高剂量组。模型组和正常组给予生理盐水灌胃，达英-35组给予达英-35灌胃，复方麦芽丸各剂量组给予相应浓度复方麦芽丸灌胃，连续21 d。ELISA检测各组大鼠血清睾酮（T）、胰岛素（INS）水平，RT-PCR检测AR、INSR基因表达。结果与正常组比较，模型组血清T、INS和AR、INSR基因表达明显升高（P＜0.05，P＜0.01）；与模型组比较，复方麦芽丸各剂量组血清 T、INS 水平和 AR、INSR基因表达明显降低（P＜0.05，P＜0.01）。结论复方麦芽丸可纠正PCOS模型大鼠的内分泌-代谢失调状态。%Objective To explore the effects of Compound Malt Pill on gene expressions of androgen receptor (AR) and insulin receptor (INSR) in polycystic ovarian syndrome (PCOS) rats; To discuss its relevant mechanism of action.Methods Letrozole was used to induce and establish rat models. Rats were randomly divided into normal group, model group, Diane-35 group, low-, medium- and high-dose Compound Malt Pill groups. The normal group and model group received normal saline by gavage; the Diane-35 group was given Diane-35 by gavage; Compound Malt Pill groups were respectively given different concentrations by gavage for 21 d. The levels of serum testosterone (T) and insulin were measured by ELISA, and the gene expressions of AR and INSR were measured by RT-PCR. Results Compared with normal group, the serum T, insulin and the gene expressions of AR and INSR in model group increased significantly (P<0.05,P<0.01); compared with model group, the serum T, insulin and the gene expressions of AR and INSR in low-, medium- and high-dose Compound Malt Pill groups decreased significantly (P<0.05, P<0.01).Conclusion Compound Malt Pill can adjust endocrine-metabolic disorder in PCOS rats.