响应面法优化复合酶提取枸杞子总黄酮的工艺研究

摘要

目的 采用响应面法优化复合酶提取枸杞子总黄酮的工艺条件.方法 在单因素试验基础上,选择酶用量、酶解时间、酶解温度为因素,进行3因素3水平的中心组合设计,采用响应面法分析3个因素对枸杞子总黄酮得率的影响.结果 复合酶辅助提取枸杞子总黄酮的工艺路线为:复合酶用量0.30%,酶解时间1.0 h,酶解温度60℃.总黄酮得率预测值为0.9492%.结论 响应面法优化复合酶辅助提取枸杞子总黄酮的方法 稳定可行,操作简单,可为深入研究及应用提供依据.%Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology (RSM).Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM.Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60℃. The predicted extraction yield of total flavonoids was 0.9492%.ConclusionUsing RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.