Ⅰ类整合子与铜绿假单胞菌多重耐药相关性研究

摘要

Objective To investigate the differences in distribution and characteristics of the class 1 integron (Int I ) between multidrug resistant (MDR) and susceptible Pseudomonas aeruginosa (PA), and to evaluate whether Int I was associated with the formation of MDR of PA. Methods Positive rate of Int I in 67 MDR and 62 susceptible isolates from clinical samples were detected by polymerase chain reaction(PCR), and integrated gene cassettes in Int I positive strains were also identified by PCR Results 32(47.76％) isolates with MDR phenotype were Int I positive but only HI. 61 %) isolate with susceptible phenotype was Int I positive, the distribution of Int I in MDR i-solates was significantly higher than susceptible ones( x2 = 36. 02,P<0. 05). The gene cassettes in MDR group carried large fragments, but the susceptible isolates carried an empty one. Conclusion Positive rate of Int I in MDR-PA is high and may be related with MDR of PA.%目的 了解Ⅰ类整合子在临床分离的铜绿假单胞菌多重耐药株及敏感株中分布的差异,初步阐明Ⅰ类整合子是否与铜绿假单胞菌多重耐药性形成相关.方法 应用聚合酶链反应(PCR)法分别检测临床分离的67株多重耐药铜绿假单胞菌和62株敏感株中Ⅰ类整合子的阳性率,并进一步用PCR证实Ⅰ类整合子阳性菌中整合子相关基因盒的存在.结果 铜绿假单胞菌多重耐药株中,32株(47.76％)为Ⅰ类整合子阳性:敏感株中仅1株(1.61％)为Ⅰ类整合子阳性,多重耐药株的Ⅰ类整合子分布显著高于敏感株(x2=36.02,P＜0.05).PCR进一步证实多重耐药铜绿假单胞菌携带Ⅰ类整合子基因盒,而敏感株携带空整合子.结论 多重耐药铜绿假单胞菌具有较高的Ⅰ类整合子阳性率,Ⅰ类整合子可能与铜绿假单胞菌的多重耐药性相关.